Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere  

作　　者：Likun Gu Zhihui Bai Bo Jin Qing Hu Huili Wang Guoqiang Zhuang HongxunZhang 

机构地区：[1]Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China [2]Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China [3]School of Earth and Environment Sciences, The University of Adelaide, Adelaide, SA 5005, Australia [4]Graduate University of Chinese Academy of Sciences, Beijing 100039, China

出　　处：《Journal of Environmental Sciences》2010年第1期134-141,共8页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.30600082,20777089);the "Knowledge Innovation" Program of Chinese Academy of Sciences (No.kzcx1-yw-06-03);the Key Technologies R&D Program of China (No.2008BADA7B01)

摘　　要：Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone library and 16S rDNA directed polymerase chain reaction with denaturing gradient gel electrophoresis （PCR-DGGE） were used for monitoring the change of bacterial community. The 16S rDNA clone library and PCR-DGGE analysis both confirmed the microbial community of wheat plant phyllosphere were predominantly of the γ-Proteobacteria phyla. Results from PCR-DGGE analysis indicated a significant change in bacterial community structure within the phyllosphere following fungicide enostroburin application. Bands sequenced within control cultures were predominantly of Pseudomonas genus, but those bands sequenced in the treated samples were predominantly strains of Pantoea genus and Pseudomonas genus. Of interest was the appearance of two DGGE bands following fungicide treatment, one of which had sequence similarities （98%） to Pantoea sp. which might be a competitor of plant pathogens. This study revealed the wheat phyllosphere bacterial community composition and a shift in the bacterial community following fungicide enostroburin application.Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone library and 16S rDNA directed polymerase chain reaction with denaturing gradient gel electrophoresis （PCR-DGGE） were used for monitoring the change of bacterial community. The 16S rDNA clone library and PCR-DGGE analysis both confirmed the microbial community of wheat plant phyllosphere were predominantly of the γ-Proteobacteria phyla. Results from PCR-DGGE analysis indicated a significant change in bacterial community structure within the phyllosphere following fungicide enostroburin application. Bands sequenced within control cultures were predominantly of Pseudomonas genus, but those bands sequenced in the treated samples were predominantly strains of Pantoea genus and Pseudomonas genus. Of interest was the appearance of two DGGE bands following fungicide treatment, one of which had sequence similarities （98%） to Pantoea sp. which might be a competitor of plant pathogens. This study revealed the wheat phyllosphere bacterial community composition and a shift in the bacterial community following fungicide enostroburin application.

关 键 词：FUNGICIDE PHYLLOSPHERE microbial community 16S rDNA clone library PCR-DGGE 

分 类 号：Q503[生物学—生物化学] S154.381[农业科学—土壤学]