RP-HPLC法同时测定人血清中氯吡格雷和阿司匹林的浓度  

Determination of Clopidogrel and Aspirin in Human Blood Serum by RP-HPLC

作　　者：王夏芹[1] 黄晓晖[2] 陈纭[2] 唐海沁[1] 郑青山[3] 金涌[2] 李俊[2] 

机构地区：[1]安徽医科大学第一附属医院心内科,合肥市230022 [2]安徽医科大学药学院,合肥市230032 [3]上海中医药大学药物临床研究中心,上海市201203

出　　处：《中国药房》2010年第2期122-124,共3页China Pharmacy

基　　金：国家自然科学基金项目(30701040);安徽省自然科学基金项目(070413117);安徽省教育厅自然科学研究项目(2006KJ307B)

摘　　要：目的:建立以反相高效液相色谱法同时测定人血清中氯吡格雷和阿司匹林浓度的方法。方法:色谱柱为Hypersil ODS C18,流动相为0.01mo·lL-1磷酸二氢钾溶液-甲醇(35∶65,磷酸调pH值至3),流速为1.0mL·min-1,检测波长为235nm,柱温为30℃。结果:氯吡格雷与阿司匹林血药浓度分别在0.25～16.0mg·L-1(r=0.9999)、0.125～8.0mg·L-1(r=0.9996)范围内线性关系良好;平均回收率分别为98.3%、99.6%,日内、日间RSD均<10%。结论:本方法简便、快速、灵敏度高,可用于人血清中氯吡格雷和阿司匹林浓度的检测与分析。OBJECTIVE： To establish an RP-HPLC method for determination of clopidogrel and aspirin in human blood serum. METHODS： The separation was performed on Hypersil ODS C18 column. The mobile phase was 0.01 mol.L^-1 potassium dihydrogen phosphate-methanol （35：65）（adjust pH to 3 by phosphoric acid）, with column temperature set at 30℃. The flow rate was 1.0 mL-min ^-1 and detection wavelength was 235 nm. RESULTS： The calibration curves were linear over the range of 0.25-16.0 mg.L^-1（r=0.999 9）for clopidogrel and 0.12548.0 mg.L^-1（r=0.999 6）for aspirin. The average recoveries were 98.3% and 99.6%, respectively. RSD of intra-day and inter-day validatin were all below 10%. CONCLUSION： The method is convenient, rapid, and sensitive for determination and analysis of clopidogrel and aspirin in human serum.

关 键 词：反相高效液相色谱法 氯吡格雷 阿司匹林 血药浓度 

分 类 号：R972[医药卫生—药品]