条石鲷烂尾白浊症病原菌的分离鉴定及系统发育分析  

Identification and phylogenetic analysis of a pathogenic bacterial strain isolated from Oplegnathus fasciatus with tail rot and whitish symptom

作　　者：闫茂仓[1,2] 单乐州[1,2] 马爱敏[1,2,3] 谢起浪[1,2] 陈少波[1,2] 邵鑫斌[1,2] 

机构地区：[1]浙江省海洋水产养殖研究所,浙江温州325005 [2]浙江省近岸水域生物资源开发与保护重点实验室,浙江温州325005 [3]山东农业大学动物科技学院,山东泰安271018

出　　处：《上海海洋大学学报》2010年第1期50-55,共6页Journal of Shanghai Ocean University

基　　金：浙江省科技厅科研院所专项公益技术攻关项目(2007F10009);浙江省科技厅重大科技攻关项目(2005C12006-02);浙江省科技厅科研院所青年人才计划项目(2006R20001);温州市科技兴海项目(S20070051)

摘　　要：从患烂尾白浊症的条石鲷肾脏和肝脏等病变组织分离出致病力较强的菌株TP0701,人工感染实验证实该菌株为条石鲷的病原菌。对该细菌进行了形态、生理生化特性测定和16S rRNA分子鉴定,测定16SrRNA基因序列,分析相关细菌相应序列的同源性,构建了系统进化树。结果表明菌株TP0701与恶臭假单胞菌(Pseudomonas putida)的亲缘关系最近,具有98.1%的同源性。结合该菌株的生理生化特性,可鉴定菌株TP0701为恶臭假单胞菌。A pathogenic bacterial strain TP0701 was isolated from red drum （Oplegnathus fasciatus） with tail rot and whitish symptom. It was proved to be the pathogen of the red drum （Oplegnathus fasciatus ） by artificial infection test. The traditional physiological and biochemical experiments were done. To investigate the phylogenetic position of this pathogen, 16S rDNA of TP0701 was sequenced and compared with that of other related strains. Molecular phylogenetic tree was constructed based on the genetic distance analysis. The results showed that strain TP0701 exhibited the highest levels of similarity to the Pseudomonas Putida. Analysizing all the results of several methods, strain TP0701 was identified as Pseudomonas Putida.

关 键 词：条石鲷 16S RRNA基因 恶臭假单胞菌 

分 类 号：S943[农业科学—水产养殖]