红毛五加叶中常春藤皂苷元的高效液相色谱法测定  

HPLC Determination of Hederagenin in Leaves of Acanthopanax giraldii Harms

作　　者：钟世红[1,2] 卫莹芳[2] 古锐[2] 吴宇[1] 段雄[2] 

机构地区：[1]成都医学院.药学院,四川成都610083 [2]成都中医药大学,四川成都611131

出　　处：《时珍国医国药》2010年第1期6-7,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金青年基金项目(No.30701087)

摘　　要：目的建立红毛五加叶中常春藤皂苷元的测定方法。方法采用高效液相色谱法。色谱柱为Welchrom C18色谱柱（4．6mm×250mm，5μm）；流动相为甲醇-水-冰醋酸-三乙胺（85：15：0．04：0．02）；流速：1．0ml·min^-1；柱温：35℃；检测波长为210nm。结果常春藤皂苷元的线性范围为0．68～10．94μg，平均加样回收率为100．42％，RSD小于2％。结论该方法准确、专属性强，可为红毛五加叶的综合开发利用提供参考。Objective To develop a determination method for the hederagenin in leaves of Acanthopanax Giraldii Harms. Methods Hederagenin was determined by HPLC method. The separation was performed on a welchrom C18 column（4.6 mm × 250 mm, 5 μm） with mobile phase of methanol - water - glacial acetic acid - triethylamine （ 85 ： 15：0.04：0.02 ）. The flow rate was 1.0 mL · min^-1. The column temperature was at 35 ℃. The detection wavelength was 210nm. Results The linear range of hederagenin was 0. 68 - 10.94 μg. The average recovery was 100.42% ,and the RSD was less than 2%. Conclusion The method is accurate and soecific which is useful for the integrative exploitation of the leaves of A. Giraldii Harms.

关 键 词：红毛五加叶 常春藤皂苷元 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]