活血熄风方对局灶性脑缺血大鼠血管内皮生长因子mRNA表达的影响  

Effects of Huoxue Xifeng Recipe on Infarction Volume and the Expressions of VEGF mRNA in Brain Tissue after Focal Cerebral Infarction in Rats

作　　者：高红莉[1] 刘昭纯[2] 

机构地区：[1]泰山医学院.药学院,山东泰安271016 [2]山东中医药大学基础医学院,山东济南250355

出　　处：《时珍国医国药》2010年第1期109-111,共3页Lishizhen Medicine and Materia Medica Research

基　　金：山东省自然科学基金(No.Y2008C144)

摘　　要：目的观察活血熄风方对局灶性脑缺血模型大鼠脑梗塞体积及脑组织中血管内皮生长因子(VEGF mRNA)表达的影响。方法W istar雄性大鼠随机分为假手术组,模型组,活血熄风方高、中、低3个剂量组(剂量分别为20,10,5 g.kg-1)和尼莫地平对照组(0.02 g.kg-1),采用光化学法建立局灶性脑缺血大鼠模型,造模前7d灌胃给药,造模后24 h取材,TTC染色法测脑梗塞体积,逆转录聚合酶链式反应(RT-PCR)法测VEGF mRNA表达。结果与模型组比较,活血熄风方各剂量组的脑梗塞体积明显减少(P<0.01),VEGF mRNA表达明显升高(P<0.01)。结论活血熄风方可明显减少局灶性脑缺血大鼠脑梗塞体积,具有脑保护作用,其作用机制可能与增强VEGF mRNA表达有关。Objective To observe the effects of Huoxue Xifeng Recipe （HXR） on infarction volume and the expressions of VEGF mRNA in brain tissue after focal cerebral infarction （ FCI ） in rats. Methods Wistar male rats were randomly divided into six groups： sham group, model group, HXR treated group（20,10,5 g·kg^-1 as high,middle and low dose respectively） and nimodipine control group（0.02 g·kg^-1）. The model of FCI was established based on the principle of photo- chemistry initiation of thrombosis, intragastric administration continued 7 days before modeling and draw the material after 24h. Infarction volume was detected by TTC staining technique, the expression of VEGF mRNA was detected by reverse transcription polymerase chain reaction（ RT- PCR）. Results The infarction volume was decreased and the expressions of VEGF mRNA was higher in HXR trerted groups than that of FCI model rats （ P 〈 0.05 or P 〈 0.01 ）. Conclusion HXR can decreased infarction volume and has a protective effect on FCI rats, and the mechanism may be related to increasing the expression of VEGF mRNA.

关 键 词：活血熄风方 光化学法 血管内皮生长因子MRNA 逆转录聚合酶链式反应 局灶性脑缺血 

分 类 号：R285.5[医药卫生—中药学]