水中痕量微囊藻毒素测定的优化  

Optimized Method for Determining Trace Levels of Microcystins in Water

作　　者：杨再荣[1,2] 董泽琴 张琳[1,2] 张维 

机构地区：[1]贵州省环境科学研究设计院,贵州贵阳550002 [2]贵州师范大学地理与环境科学学院,贵州贵阳550001 [3]贵州省水污染控制与资源化技术研究重点实验室,贵州贵阳550002

出　　处：《环境科学与技术》2010年第2期118-121,共4页Environmental Science & Technology

基　　金：贵州省社会发展科技攻关项目(黔科合S字[2007]1007号);贵州省优秀科技教育人才省长专项资金项目(黔省专合字[2007]108号);贵州省高层次人才科研条件特助经费项目资助(TZJF-2007-15)

摘　　要：文章对固相萃取(SPE)-高效液相色谱法(HPLC)测定微囊藻毒素过程中的定量参数、固相萃取柱的吸附能力、样品浓缩等进行了研究。结果表明,峰高度比峰面积在定量方面具有更好的稳定性。影响富集最大的因素是流速而不是富集次数;在藻毒素含量达到126.3μg时,仅有3.4%的藻毒素透过C18固相萃取小柱;蒸发皿蒸发可代替通用的旋转蒸发,从而优化藻毒素的测定。This paper deals with trace determination of microcystins in water using HPLC, which involves pretreatment with solid-phase extraction （SPE） columns. Adsorption capacity of the solid phase extraction cartridges, condensation of water sample and some of the relative quantitative parameters are investigated. Result shows that peak height is of more quantitative stability than that of peak area, and the main factor affects enrichment is velocity rather than times for enrichment. To facilitate pretreatment of samples, it is suggested to use dish evaporating method in place of the common rotation evaporation.

关 键 词：高效液相色谱 微囊藻毒素 测定优化 

分 类 号：X832[环境科学与工程—环境工程]