山西省主推玉米杂交种纯度鉴定SSR核心引物的筛选  

Selection of SSR Core Primers for Maize Hybrid Purity Identification in Shanxi Province

作　　者：王陆军[1] 白建荣[1] 王艳梅[1] 王燕[2] 赵文博[2] 苏亮[2] 

机构地区：[1]山西省农业科学院作物遗传研究所,山西太原030031 [2]山西大学生物工程中心,山西太原030006

出　　处：《山西农业科学》2010年第1期19-22,共4页Journal of Shanxi Agricultural Sciences

基　　金：山西省科技攻关项目(051006);山西省科技基础条件平台建设项目(051014)

摘　　要：选用10对SSR核心引物对18份山西省玉米杂交种进行DNA指纹分析,并综合考虑多态性和杂合率,认为umc1590和umc1196这2对引物可鉴定17个品种纯度,phi402893,phi102228,phi233376,p-phi072,umc1066和phi022也可作为纯度鉴定的引物。建议采用umc1590,phi402893,phi233376,phi022这4对引物对18个杂交种纯度进行检测。18 maize hybrids were analyzed with 10 SSR core primers. According to the polymorphism and heterogosite rate, 17 maize hybrids could be identified with umc1590 and umc1196,and phi402893, phi102228,phi233376,p-phi072,umc1066 and phi022 could also be thought as suitable primers for identifying maize hybrids. Four primers were suggested to identify 18 maize hybrids.

关 键 词：玉米 SSR 纯度鉴定 核心引物 

分 类 号：S513[农业科学—作物学]