当归对宫内缺氧新生大鼠大脑皮质神经元与VEGF mRNA表达的影响  

Effect of Angelica on Neurons and the Expression of VEGF mRNA in the Cerebral Cortex of Neonatal Rats Following Intrauterine Hypoxia

作　　者：梅欣明[1] 刘敦玉[2] 赵峰[1] 马晶[1] 余鸿[1] 

机构地区：[1]泸州医学院组胚教研室,四川泸州646000 [2]泸州医学院附属中医院急诊科,四川泸州646000

出　　处：《四川动物》2010年第1期116-119,共4页Sichuan Journal of Zoology

基　　金：四川省科技厅课题(05JY029-103);四川省教育厅课题(2006A152)

摘　　要：目的探讨宫内缺氧对新生大鼠大脑皮质神经元与VEGF mRNA表达的影响以及当归的调控作用。方法孕14d健康SD雌性大鼠15只,随机分为对照组、缺氧组和当归组各5只,于孕14d开始将当归组与缺氧组孕鼠置于低张氧浓度三气培养箱中,制作胎鼠宫内缺氧模型,此前一小时按8mL/kg分别给予当归和生理盐水尾静脉注射,对照组不缺氧,余同缺氧组。三组孕鼠分娩当日每窝随机选取新生鼠4只,取脑组织多聚甲醛固定,石蜡包埋切片、NSE mRNA、VEGF mRNA原位杂交,400倍拍照、IPP6.0软件图像分析。结果缺氧组新生大鼠大脑皮质NSE mRNA阳性细胞数较对照组减少,积分光密度值(IOD)降低(P<0.05),VEGF mRNA阳性细胞IOD值升高(P<0.05);当归组新生大鼠大脑皮质NSE mRNA阳性细胞数较缺氧组增多、IOD值增高(P<0.05),VEGFmRNA阳性细胞IOD值增高(P<0.05)。结论宫内缺氧可致新生大鼠大脑皮质神经元受损,当归注射液对此损伤有一定保护作用,其机制可能是通过上调大脑皮质VEGF mRNA的表达而使缺氧环境改善。Objective To explore the effect of intrauterine hypoxia on the neurons and the expression of VEGF mRNA in the cerebral cortex of neonatal rats, and the regulative role of angelica to them. Method Fifteen pregnant healthy SD rats were divided randomly into three groups： a control group（ CG）, a hypoxia group （HG） and an angelica group （AG） , with each group containing 5 rats. The angelica group and hypoxia group pregnant rats were placed in a three-gas incubator from the start of pregnant 14^th days after to produce the intrauterine hypoxia model of fetal rats, and one hour after they were removed from the incubators, the pregnant rats received injections of angelica and normal saline （8 mL/kg） through the caudal vein. The control group procedures were identical to the hypoxia group, but under normal, non-hypoxic conditions. After birth, four neontal rats were selected randomly from each litter and brain tissue was sampled and fixed in paraformaldehyde, embedded in paraffin and sectioned for NSE mRNA and VEGF mRNA in situ hybridization （ISH）. Photos of the cerebral cortex were taken under a microscope at 400 x. The photos were analyzed with IPP6.0 software. Results The quantity and integral optical density （IOD） value of positive NSE mRNA cells in the HG were obviously lower than that of the CG（ P 〈 0.05）, and the IOD value of positive VEGF mRNA cells in the HG was significantly higher than that of the CG （P 〈 0.05 ） ; but the quantity and IOD value of positive NSE mRNA cells in the AG were higher than that of the HG（ P 〈 0.05 ）, and the IOD value of positive VEGF mRNA cells in the AG was significantly higher than that of the HG（P 〈0.05）. Conclusion Intrauterine hypoxia could damage the neurons in the cerebral cortex of neonatal rats, and angelica injections could play a protective role to a certain degree by possibly upregulating the expression of VEGF mRNA in the cerebral cortex to ameliorate the intrauterine hypoxic environment.

关 键 词：皮质 神经元 VEGF 当归 缺氧 

分 类 号：S853.7[农业科学—临床兽医学]