补肾活血中药对系膜增生性肾炎大鼠MMP-2表达的影响  

Effects of nourishing kidney and activating blood flow on expression of matrix metalloproteinase-2 in mesangial proliferative glomerulonephritis rat

作　　者：张勉之[1] 张大宁[2] 赵松[3] 张艳秋[1] 刘淑霞[3] 王丽敏[1] 

机构地区：[1]天津市公安医院肾内科,天津300042 [2]天津市中医药研究院肾内科,天津300020 [3]河北医科大学病理教研室,石家庄050017

出　　处：《中华中医药杂志》2010年第1期114-117,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：天津市卫生局中医;中西医结合专项资金课题(No.2005061)~~

摘　　要：目的:研究补肾活血中药对系膜增生性肾小球肾炎的基质金属蛋白酶-2(MMP-2)表达的影响。方法:取雄性SD大鼠54只,实验设正常对照组、模型组、补肾活血药物干预组,观察MMP-2的表达。结果:通过免疫组化、流式细胞术、免疫蛋白印记以及RT-PCR等方法表明模型组大鼠MMP-2表达较正常对照组明显减少,经补肾活血中药治疗后,MMP-2活性可部分恢复。结论:补肾活血中药能上调MMP-2的表达及活性的部分恢复,使得胶原合成减少,在延缓肾小球疾病的进展,防治肾小球硬化方面具有确切疗效。Objective： To investigate the expression of mmp-2 in mesangial proliferative glomerulonephritis（MsPGN） rat renal tissue and the effects of nourishing kidney and activating blood flow recipe of TCM. Methods： 54 Male SD rats were divided into control group, MsPGN group and treatment group with nourishing kidney and activating blood flow recipe of TCM. Immunohistochemical staining, flow cytometry, western blot and RT-PCR were used to check the protein expression of romp-2. Results： In MsPGN group, following with the disease progressing, the glomerulus grew graduatly, ECM increased, the basement membrane of glomerulus was thicker. The immunohistochemical staining showed that the chief positive granules were deposited in glomerulus and renal tubular, the expression of mmp-2 was lower than that in control group, but in treatment group the expression of mmp-2 was higher than that in MsPGN group. Conclusion： In MsPGN renal tissue, the expression of mmp-2 was reduced, its activity was weakened. Nourishing kidney and activating blood flow recipe of TCM could induce the expression of MMP-2 and partly recover the activity. Nourishing kidney and activating blood flow recipe of TCM could resist glomerular sclerosis and postpone the course of chronic renal failure.

关 键 词：系膜增生性肾小球肾炎 基质金属蛋白酶-2 补肾活血法 

分 类 号：R285.5[医药卫生—中药学]