优化农杆菌介导基因转化效率因子分析  

Analysis of the Optimized Factors on the Efficiency of Agrobacterium Mediated Gene Transformation

作　　者：刘彩萍[1] 曾炳山[1] 裘珍飞[1] 李湘阳[1] 刘英[1] 

机构地区：[1]中国林业科学研究院热带林业研究所繁育中心,广东广州510520

出　　处：《安徽农业科学》2010年第3期1141-1143,1202,共4页Journal of Anhui Agricultural Sciences

基　　金：国家林业局"948"引进国际先进农业科学技术项目(2006-4-70)

摘　　要：共培养法是农杆菌介导转基因比较常用的方法之一。建立一个高效的遗传转化体系,除了确定抗生素种类,菌种和菌液浓度,预培养和共培养时间等基本因子外,优化影响转化的因子非常重要。主要介绍和分析共培养法中可能优化的影响基因转化因子,为提高农杆菌介导转化率提供了方法和依据。Co-culture is one of the Agrobacterium mediated gene transformation methods. In addition to the choice of antibiotics, Agrobacterium strain and optical density, time of the pre-culture and co-culture, optimization of factors affecting transformation are also very important in developing an efficient genetic transformation protocol. The optimized factors were introduced in co-culture method in order to provide methods and basis for enhancing the efficiency of Agrobacterium mediated transformation.

关 键 词：农杆菌 预培养 侵染液 接种 共培养 

分 类 号：S188[农业科学—农业基础科学]