青春期Beagle犬雌性生殖器官形态特征及其首次发情个体差异  

HISTOLOGICAL CHARACTERS OF FEMALE REPRODUCTIVE ORGANS AND THE INDIVIDUAL DIFFERENCES OF THE FIRST ESTRUS CYCLE IN PUBERTAL FEMALE BEAGLE DOG

作　　者：华晓萍[1,2] 杜艳春[1,2] 邱爽[1,2] 陈珂[1,2] 张银柱[3] 李红霞[1,2,3] 岑小波[1,2,3] 王莉[1,2] 胡春燕[1,2] 

机构地区：[1]四川大学华西医院.国家成都中药安全性评价中心,成都610041 [2]成都华西海圻医药科技有限公司 [3]四川大学公共卫生学院

出　　处：《现代预防医学》2010年第2期309-312,共4页Modern Preventive Medicine

基　　金：国家科技重大专项"规范化药物安全评价技术平台的建设"(2008ZX09305);国家科技支撑计划"药物安全关键技术研究"(2006BAI14B06)资助

摘　　要：[目的]了解青春期中期雌性Beagle犬的卵巢、子宫、阴道、乳腺在不同动情周期的组织学形态及其首次发情的时间差。[方法]取20只10-11月龄雌性Beagle犬,麻醉后失血安乐死,取卵巢、子宫、阴道和乳腺,10%中性缓冲福尔马林固定后制成H＆E切片,光镜下观察。[结果]所有犬只均已进入首次发情周期,40%处于动情前期,10%处于动情期（动情后期）,50%处于动情间期,无动物处于不动情期（休情期）。[结论]试验周期较短的长期毒性试验,应尽量使用月龄超过10个月的Beagle犬;评价性激素样作用药物应考虑动物年龄及个体差异对性成熟时间及周期的影响。[Objective] To understand the histological characters in uterus,ovaries,vagina and mummy gland of the pubertal Beagle dog,and the difference in initial time of the first sex cycle.[Methods] 20 female Beagle dogs in age of 1011monthes were euthanized followed by exsanguinations and necropsy.Uterus,ovaries,vagina and mummy gland were collected,fixed in 10% neutral buffered formalin,sectioned,stained with HE,and evaluated by microscope.[Results] All the dogs were in their first estrus cycle,40% dogs were in proestrus,10% dogs were in estrus（metestrus）,50% dogs were in diestrus;and no dog was in anestrus.[Conclusion] Older than 10 month Beagle dog suggested to be used in short term toxicological study;age and the individual difference should be considered when evaluation of compound is conducted in female dogs,especially for those hormonal effects.

关 键 词：BEAGLE犬 青春期中期 动情周期 组织学特点 个体差异卵巢 子宫 阴道 乳腺 

分 类 号：R711.1[医药卫生—妇产科学]