黑木耳总DNA提取及PCR-ISSR引物筛选  

DNA Extraction and ISSR Primer Screening of Auricularia auricula

作　　者：曾晓丽[1] 汪思迪[1] 程水明[1] 

机构地区：[1]黄冈师范学院生命科学与工程学院,湖北黄冈438000

出　　处：《湖北农业科学》2009年第12期2926-2928,共3页Hubei Agricultural Sciences

基　　金：黄冈师范学院大学生科研基金项目

摘　　要：以14个黑木耳[Auricularia auricula(L.ex Hook)Underw]菌株为试材,对黑木耳总DNA提取方法和ISSR-PCR扩增反应条件及引物进行了筛选。结果表明,改进的SDS抽提法可以在较短时间内提取到高纯度的DNA产物;并筛选出了可产生遗传多样性的15条引物,其中引物ISSR11、ISSR12、ISSR14扩增结果的稳定性好、多态性强。经凝胶电泳比较,引物ISSR14可作为黑木耳PCR–ISSR反应的适宜引物。The methods of DNA extration, primers of ISSR and rection conditions were selected using fourteen kinds of Auricularia auricular as experiment materials. The results showed that the improved SDS method could obtain pure DNA in short time. Fifteen primers of genetic variation were selected. Primers ISSR11、ISSR12、ISSR14 could produce stable and intense polymorphism. Primer ISSR14 could be used as the suitable primer for A. auricula variety.

关 键 词：黑木耳 内部简单重复序列分子标记 DNA提取 引物筛选 

分 类 号：S646.6[农业科学—蔬菜学] Q78[农业科学—园艺学]