利川马mtDNA Cytb基因遗传多态性分析  

Analyses on mtDNA Cytb Genetic Diversity in Lichuan Horse

作　　者：秦芳[1] 王小斌[1] 张云生[1] 张妙李 李晓锋[3] 雷初朝[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]湖北省恩施自治州畜牧技术推广站,湖北恩施445000 [3]湖北省农业科学院畜牧兽医研究所,武汉430209

出　　处：《湖北农业科学》2009年第12期2935-2937,共3页Hubei Agricultural Sciences

基　　金：教育部新世纪优秀人才支持计划项目(NCET-07-0699);西北农林科技大学科研专项(07ZR004)

摘　　要：利用PCR和生物信息技术,对22匹利川马线粒体DNA Cytb基因全序列的遗传多态性及系统进化进行了分析。结果发现,利川马的Cytb基因全序列为1140bp,并且检测到9种单倍型和26个核苷酸多态位点,约占所测核苷酸总长的0.53%。利川马mtDNA Cytb基因单倍型多样度为0.8400,核苷酸多样度为0.0486,表明利川马mtDNA Cytb基因遗传多态性较丰富。根据mtDNA Cytb基因序列构建的NJ树,发现利川马是多起源的物种。The genetic diversity and phylogenetic evolution of mitochondrial DNA （mtDNA） Cytb sequences in 22 Lichuan horse samples were analyzed by PCR and bioinformation techniques. The results revealed that the complete Cytb sequence was 1 140 bp, and 9 mitochondrial haplotypes with 26 polymorphie sites which account for 0.53% of the analyzed length of the 1 140 bp were detected. The haplotype diversity and nucleotide diversity estimated were 0.840 0 and 0.048 6, respectively, showing rich mitochondrial genetic diversity in Lichuan horse breed. The constructed Neighbor-Joining phylogenetic tree based on the mtDNA Cytb haplotypes, showed that in Lichuan horse was a species with multiple maternal origins.

关 键 词：利川马 MTDNA CYTB基因 遗传多态性 起源 

分 类 号：S821.89[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]