蚕豆锈病的离体叶片人工接种技术研究及微生物源先导化合物筛选  

Studies on Artificial Inoculation of Broadbean Rust on Leaf-dish and Screening of Lead Compounds from Microorganism

作　　者：张志刚[1] 杨自文[1] 王开梅[1] 张亚妮[1] 刘翠君[1] 江爱兵[1] 梁英[1] 

机构地区：[1]湖北省生物农药工程研究中心/湖北省农业科技创新中心生物农药研究分中心,武汉430064

出　　处：《湖北农业科学》2009年第12期3037-3040,共4页Hubei Agricultural Sciences

基　　金：湖北省十一五重大科技攻关项目(2006AA205A01);湖北省农业科技创新中心资助项目

摘　　要：蚕豆锈病由蚕豆单孢锈菌(Uromyces fabae de Bary)引起,是一种常见专性寄生的真菌病害,其侵染和传播方式与大豆锈病(Phakopsora pachyrhiri Syd.)相同,主要以夏孢子传播。因此常作为大豆锈病研究的模式靶标。又因其无法在人工培养基上生长繁殖,而基于植株的筛选方式难以适应先导化合物筛选。该文针对蚕豆锈病离体叶片人工接种技术进行了探索,对影响蚕豆锈病侵染的主要因素进行了研究,建立了基于离体叶片法筛选的先导化合物筛选方法。采用离体叶片孢子悬浮液喷雾法对1522份真菌固体发酵提取物和放线菌液体摇瓶发酵提取物进行了初筛,筛选出具有杀菌活性的提取物90份。对经过HPLC纯化后的540个组分进行复筛,获得具较高杀菌活性的化合物3个。筛选结果表明该筛选方法能稳定、高效地进行先导化合物筛选。Broadbean rust is a kind of obligateparasite. The methods of infecting and spreading are similar with soybean rust （Phakopsora pachyrhizi Syd）.So it is always used as the model target of soybean rust. The culture of this pathogen is not progressing in media, so the screening of lead compounds confined in plants. The artificial inoculation of broadbean rust had been studied on leaf-dish. The factors of effect on infection of broadbean rust had been studied. The program of screening method was found based on method of the leaf-dish,1 522 extracts from microorganism had been tested by the screening method, 90 extracts showed fungicidal activity. 540 samples formulated from profiling by HPLC had been tested, and their lead compounds had been found. The screening results showed that the screening method was stable and efficient.

关 键 词：蚕豆锈病 人工接种 先导化合物 杀菌活性 叶片法筛选 

分 类 号：S436.43[农业科学—农业昆虫与害虫防治]