微分离方法原代培养大鼠肾近曲小管上皮细胞  

Primary culture of renal proximal convoluted tubular epithelial cells in rats by microdissection

作　　者：余德芊[1] 王富英[2] 马元芬[3] 刘晓红[1] 秦伟[1] 高原[4] 

机构地区：[1]遵义医学院生理学教研室,贵州遵义563003 [2]惠州卫生学校检验教研室,广东惠州516002 [3]遵义医学院形态学实验室,贵州遵义563003 [4]遵义医学院珠海校区生理学教研室,广东珠海519041

出　　处：《遵义医学院学报》2009年第5期448-450,共3页Journal of Zunyi Medical University

基　　金：珠海市科技基金资助项目(PC20052031)

摘　　要：目的建立比较理想的大鼠肾近曲小管上皮细胞的原代培养及鉴定方法。方法采用显微微分离单根肾近曲小管节段的方法进行原代培养,并用免疫细胞化学方法和电镜进行鉴定。结果用微分离方法成功地培养出肾小管上皮细胞,经鉴定为该细胞。结论微分离方法是大鼠肾近曲小管上皮细胞原代培养的有效方法。Objective To establish a practical method for primary euhure and identification of rat renal tubular epithelial cells. Methods Primary culture of renal proximal convoluted tubular epithelial cells by microdissection. The cell types were idenfied by immunocytochemistry and electron mieroseope. Results The method for primary cuhure was successful, so was the identification of the cultured cells. Conclusions The present method is an efficient means for the primary culture and identification of rat renal tubular epithelial cells.

关 键 词：肾小管上皮细胞 微分离 原代培养 

分 类 号：Q813.11[生物学—生物工程]