正交实验法优选复方中药中芍药苷的提取工艺研究  

STUDY ON OPTIMUM EXTRACTION PROCESS FOR PAEONIFLORIN IN COMPOUND CHINESE HERBAL MEDICINE BY ORTHOGONAL EXPERIMENTAL DESIGN

作　　者：丁呈华[1] 曹丰璞[1] 柳文敏[1] 李聪聪[1] 

机构地区：[1]南阳师范学院,河南南阳473061

出　　处：《南阳理工学院学报》2009年第4期64-66,70,共4页Journal of Nanyang Institute of Technology

基　　金：南阳市科技局项目(2007S1310)

摘　　要：采用正交实验的方法,以芍药苷为考察目标,用反相高效液相色谱法(HPLC)测定复方中药中芍药苷的含量,考察了乙醇浓度、乙醇用量、提取时间和提取次数4个因素。结果表明,最佳提取工艺为A2B1C1D3,即80%的乙醇8倍量,回流提取3次,每次1小时。By orthogonal experimental design, the content of Peoniflorin in compound Chinese herbal medicine is measured by reversed-phase high-performance liquid chromatography. Alcohol concentration, solvent amount, extraction times and extraction time as factors are analyzed. The results show that the optimum extracting condition is A2B1C1D3 , that was 80% alcohol, eight times amount,refluxing for 3 times and 1 h each time.

关 键 词：正交实验 复方中药 芍药苷 反相高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]