Chelex~100提取果蝇非新鲜标本核基因组方法的建立  

The Foundation of Chelex ○R 100 Method to Extract the Genome of Drosophila Non Fresh Materials

作　　者：马云梅[1] 吕静[1] 戴灼华[1] 

机构地区：[1]北京大学生命科学学院

出　　处：《北京大学学报（自然科学版）》1998年第6期841-843,共3页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：国家自然科学基金

摘　　要：引言从大量材料中提取ＤＮＡ的经典方法包括分离和纯化的过程，繁琐的操作步骤会引起提取物的污染。Ｃｈｅｌｅｘ○Ｒ１００是一种弱的阳离子螯合树脂，可以螯合一些被认为在高温、低离子强度下催化ＤＮＡ降解的金属离子［１］，在提取ＤＮＡ过程中防止ＤＮＡ的降解。此外...The genome of Drosophila immigrans non fresh materials (soaked in 70% & 100% ethanol and dried materials) was extracted with Chelex ○R 100.Then the ITS1 fragment which is between 18S rRNA and 5.8S rRNA was amplified with primers designed by our laboratory.The amplified fragment was linked to pGEM T easy Vector (Promega company) and the final plasmid was transformed to E.coli DH5α.Then the plasmid was extracted and digested with endonuclease.According to the result of one of the non fresh materials,the effects that templates were prepared using the “Chelex method” and the usual method were the same.Furthermore,the pollution and DNA waste were reduced in the “Chelex method”.

关 键 词：果蝇 非新鲜标本 ITS1 Chelex 基因组 提取 

分 类 号：Q523.03[生物学—生物化学] Q781