苍耳粗蛋白生物活性的研究(英文)  

STUDY ON THE BIOLOGICAL ACTIVITIES OF CRUDE PROTEINS FROM XANTHIUM SIBIRICUM

作　　者：王淑芳[1] 王勇[1] 王宁宁[1] 田俊英[1] 朱亮基[1] 李刚[2] 

机构地区：[1]南开大学生物化学及分子生物学系,天津300071 [2]南开大学分子生物研究所,天津300071

出　　处：《南开大学学报（自然科学版）》1998年第3期68-74,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

摘　　要：采用不同饱和度硫酸铵盐析法进行不同生长发育时期苍耳叶片和种子粗蛋白的提取及分级分离，测定其对酵母菌生长、ATPase活性和线粒体呼吸的抑制作用．结果表明：50％和80％饱和度硫酸铵环境中析出的成熟种子粗蛋白的抑制效应最为显著．Crude proteins were extracted from blades and seeds of Xanthium silericum in different growth and development stage by using salting-out effect of ammonium sulfate of different saturations. The inhibitory effects of crude proteins on the growth of yeast, activity of ATPase and mitochondrial respiration were investigated. The results indicate that the inhibitory effect of crude proteins precipitated with ammonium sulfate of 50% or 80% saturation from ripe seeds is most remarkable.

关 键 词：苍耳 粗蛋白 ATP酶 线粒体呼吸 生物活性 

分 类 号：Q949.783.5[生物学—植物学]