双虾标青草油微生物限度检查方法的建立  

Establishment of Method for Microbial Limit test of Shuangxiabiao Qingcao Oil

作　　者：隗秀荣[1] 范震洪[2] 

机构地区：[1]北京市第六医院,北京100007 [2]北京天坛医院

出　　处：《中国药事》2009年第12期1213-1215,共3页Chinese Pharmaceutical Affairs

摘　　要：目的建立双虾标青草油微生物限度检查方法。方法采用薄膜过滤并在冲洗液中加入增溶剂的方法,去除双虾标青草油的抗菌活性。结果满足《中国药典》2005版验证试验的基本要求。5株验证菌株中金黄色葡萄球菌对双虾标青草油最敏感,可作为双虾标青草油微生物限度检查方法的质控菌株。结论该方法可作为双虾标青草油的常规微生物限度检查方法。Objective To establish a method for microbial limit test of Shuangxiabiao qingcao oil.Method The filtration method was used to inhibit the residual antimicrobial activity when the isopropyl myristate was firstly used as solvent and then the solubilizer was added into the solution.Result The method can pass the validation test of Chinese Pharmacopoeia 2005 version.The Staphylococcus aureus is the most sensitive strain among the five validation strains,which is recommended as positive control in experiments.Conclusion The method has been used in microbial limit test for Shuangxiabiao qingcao oil preparation control.

关 键 词：双虾标青草油 薄膜过滤 增溶剂 质控菌株 

分 类 号：R927.33[医药卫生—药学]