Meso-四(4-甲氧基-3-磺酸苯基)卟啉与钴的显色反应及其钴的价态研究  

Study on the Spectrophotometric Determination of Cobalt with Meso T(4 MO 3 SP)P and Its Valence Species

作　　者：陈秀华[1] 蔡国权[1] 

机构地区：[1]杭州大学化学系

出　　处：《杭州大学学报（自然科学版）》1998年第4期59-62,共4页Journal of Hangzhou University Natural Science Edition

摘　　要：本文研究了在弱酸性介质中，Ｃｏ（Ⅱ）与Ｔ（４ＭＯ３ＳＰ）Ｐ的显色反应．当溶液ｐＨ为４．１５时，显色剂和配合物的最大吸收峰分别在４４０．０ｎｍ和４２７．０ｎｍ．显色反应完全后，经酸化，ｐＨ为２．６５，配合物不分解，最大吸收峰不位移．实验证明，钴以二价态参与反应．Ｃｏ（Ⅱ）与Ｔ（４ＭＯ３ＳＰ）Ｐ的配位比为１∶１，Ｃｏ（Ⅱ）含量在０～３．０μｇ／２５ｍＬ范围内符合比耳定律，摩尔吸光系数ε＝１．９５×１０５Ｌ·ｍｏｌ－１·ｃｍ－１，回归方程ｙ＝０．００９９３３＋０．１２２２ｘ，相关系数ｒ＝０．９９９１．实验测定ＶＢ１２针剂中Ｃｏ（Ⅱ）含量。The condition of the color reaction meso T(4 phexamethoxy 3 sulfophonyl)porphyrin with cobalt were studied at pH4.15.It has been shown that the maximum absorption wavelengths of the reagent and the complex respectively are 440nm and 427nm.After color reaction finished,the system was acidify to pH 2.63,the complex can not be resolved.The results show that the ion of reaction with porphyrin is cobalt(Ⅱ).The coordination ratio of Co(Ⅱ) to T(4 MO 3 SP)P is 1∶1.The apparent molar absorption coefficient is 1.95×10 5L·mol -1 ·cm -1 . Beer's law is obey in concentration range of 0～3.0μg/25mL.This method has been applied to the determination of micro Co(Ⅱ) in vitamin B 12 .The results are favorable agreed with that obtained by AAS.

关 键 词：钴 卟啉 光度法 显色反应 价态 磺酸苯基 

分 类 号：O614.812[理学—无机化学] O657.3[理学—化学]