施万细胞高效体外传代培养的实验研究  

Experimental Study on Efficient Serial Subcultivation of Schwann Cells in Vitro

作　　者：迟建光 郝伟[2] 姜明[2] 王福利[3] 刘建平[3] 董青川[3] 

机构地区：[1]山东莱州市中医院,山东莱州261400 [2]解放军济南军区总医院,山东济南250031 [3]解放军第四军医大学西京医院,陕西西安710032

出　　处：《临床军医杂志》2009年第6期946-948,F0004,共4页Clinical Journal of Medical Officers

摘　　要：目的探讨获取大量高纯度施万细胞（SC）的体外培养纯化方法。方法取6-7 d SD乳鼠坐骨神经,立体显微镜下机械性剥除神经外膜与束膜,采用低浓度胰酶消化法和差速贴壁法去除成纤维细胞,阿糖胞苷与G enetic in联合应用进行SC纯化,牛脑垂体提取物（BPE）促进SC增殖。结果经形态学及S-100蛋白相关抗原免疫细胞化学染色鉴定证实所获得的SC状态良好,纯度较高,可达96%以上。结论本方法可以体外获取大量、高纯度的SC,为神经组织工程的研究奠定了实验基础。Objective To obtain an efficient method for culturing highly purified and large amount of Schwann cells（SCs） in vitro.Methods The sciatic nerves were removed aseptically under anatomical microscope from 6 to 7 days old Sprague-Dawley rats.Low concentration enzymatic dissociation and differential adhesion methods were used to eliminate fibroblasts.SCs were purified by Cytosine arabinoside and geneticin and proliferated by bovine pituitary extract（BPE）.Results SCs were identified by SABC immunohistochemistry methods with rabbit anti-S100 protein antibody.The percentage of purified SCs was over 90% and in a good state.Conclusion This method harvests large amount of SCs with high purity and establishes experimental groundwork for nerve tissue engineering.

关 键 词：施万细胞 体外培养 纯化 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]