猪腹部火器伤肠管穿透后NF-κB在心脏中的表达及其意义  

Changes in Cardiac NF-κB Activity and Their Significance after Intestinal Perforation due to Abdominal Firearm Wound

作　　者：薛会朝[1] 刘江伟[2] 李泽信[1] 冯德元[2] 卢开柏[2] 李建英[2] 

机构地区：[1]新乡医学院第一附属医院普外科,河南卫辉453100 [2]解放军兰州军区乌鲁木齐总医院肝胆外科,新疆乌鲁木齐830000

出　　处：《临床军医杂志》2009年第6期949-951,F0004,共4页Clinical Journal of Medical Officers

基　　金：兰州军区医药卫生科研基金资助项目(LXH-2005019)

摘　　要：目的探讨NF-κB在腹部火器伤肠管穿透后心脏中的变化及意义。方法健康长白仔猪42头随机等分为对照组和伤后1、2、4、8、12 h和24 h组,实验组建立腹部火器伤肠管穿透模型后,用免疫组化图像分析法测定各组心肌组织NF-κB活性,同时测定血清中LDH、CK-MB水平,在光镜下观察各组心脏组织学变化,电镜下观察心脏超微结构改变。结果伤后各组心脏NF-κB表达明显高于对照组。伤后各组血清LDH、CK-MB水平均高于对照组。伤后8、12、24 h组光镜下出现逐渐加重的心肌细胞水肿、变性;电镜下4、8、12、24 h组出现逐渐的线粒体肿胀、溶解;对照组光、电镜下未见明显的损伤性变化。相关分析表明,伤后心肌组织NF-κB表达与血清LDH、CK-MB水平均呈正相关(r值分别为0.968、0.957,P<0.05)。结论腹部火器伤肠管穿透后心肌组织内NF-κB活性增强;NF-κB在腹部火器伤肠管穿透后继发性心脏损伤中具有重要意义。Objective To investigate the changes in cardiac NF-κB activity and their significance in heart injury after intestinal perforation due to abdominal firearm wound.Methods A total of 42 Chang-Bai piglets were randomized into seven groups,i.e.control group and wounded 1-hour group,2-hour group,4-hour group,8-hour group,12-hour group and 24-hour group.The models of intestinal perforation due to abdominal firearm wound were established in the wounded groups.Cardiac NF-κB activity was measured with immunohistochemical staining and microscopical examination in all the groups.Levels of serum LDH and CK-MB were determined.The alterations of cardiac tissue were observed under light microscope and the ultrastructural changes of heart under electron microscope.Results The expression of cardiac NF-κB in the wounded groups was significantly elevated compared to the control group,as well as the levels of serum LDH and CK-MB.The experimental groups of 8-,12-and 24-hour appeared gradually aggravated cardiac cell edema and degeneration under light microscope,and the mitochondria appeared gradually aggravated swelling and dissolved under electron microscope in the groups of 4-,8-,12-and 24-hour.There were no obviously changes in the control group.In addition,the expression of cardiac NF-κB was positively correlated with serum LDH or CK-MB levels（r=0.968,0.957,P〈0.05）.Conclusion After intestinal perforation due to abdominal firearm wound,cardiac NF-κB activity increases;NF-κB may play an important role in the subsequent heart injury.

关 键 词：NF-ΚB 腹部火器伤 心脏损伤 多器官功能障碍综合征 

分 类 号：R826.5[医药卫生—临床医学]