重组hFGF-7腺病毒对角质形成细胞的生物学效应  

The biological effects of recombinant hFGF-7 adenovirus on keratinocytes

作　　者：吴晓萍[1] 曾耀英[1] 

机构地区：[1]暨南大学生命科学技术学院组织移植与免疫实验中心,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2009年第6期636-639,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家高技术研究发展计划项目863计划项目(2007AA02Z110);广东省自然科学基金项目(9151064001000031)

摘　　要：目的:研究重组腺病毒导入人成纤维细胞生长因子7(hFGF-7)对人皮肤角质形成细胞(HaCat)的生物学效应。方法:通过倍比稀释和感染实验,测定重组腺病毒rAd-hFGF-7的滴度;流式细胞术检测重组腺病毒感染HaCat细胞的感染效率;MTT法检测rAd-hFGF-7对HaCat细胞增殖的影响;重组腺病毒对HaCat细胞周期的影响通过流式细胞术进行检测。结果:高滴度重组腺病毒可高效感染HaCat细胞,其促细胞增殖作用随着重组腺病毒MO I值的增加而增强,当MO I值为50时,感染细胞进入S期和G2期的细胞比率显著增加。结论:重组腺病毒rAd-hFGF-7可促进HaCat细胞的增殖,改变HaCat细胞周期。Aim：To investigate the biological effects of recombinant adenovirus rAd-hFGF-7 on keratinocytes. Methods： The titer of recombinant adenovirus was assessed by doubling dilutions and infection experiments. Flow cytometry was applied to detect the infection efficacy of recombinant adenovims infecting HaCat cells. The influences of the recombinant adenovirus on the proliferation and cell cycle of HaCat cells were checked by MTT and flow cytometry respectively. Results： The recombinant adenovirus rAd-hFGF-7 could effectively infect HaCat cells. Enhanced stimulation to cell proliferation was observed in HaCat cells infected with increasing MOI of recombinant adenovirus. Cell ratio in S phase and G2 phase was increased when HaCat cells was infected with rAd-hFGF-7 （MOI of 50）. Conclusion：The recombinant adenovirus rAd-hFGF-7 could promote cell proliferation and change cell cycle of HaCat cells.

关 键 词：成纤维细胞生长因子7 腺病毒 细胞增殖 细胞周期 

分 类 号：Q786[生物学—分子生物学]