万寿菊属品种资源遗传关系的ISSR分析  

Analysis of Genetic Relatedness of Genetic Resources of Tagetes as Revealed by ISSR

作　　者：曾丽[1,2] 赵梁军[1] 孙佳[2] 赵子刚[2] 杨帆[2] 

机构地区：[1]中国农业大学观赏园艺与园林系,北京100193 [2]上海交通大学农业与生物学院,上海200240

出　　处：《中国农业科学》2010年第1期215-222,共8页Scientia Agricultura Sinica

基　　金：国家"863"计划项目(2007AA10Z189)

摘　　要：【目的】通过ISSR分子标记研究品种资源遗传关系,探讨其种群遗传多样性水平和遗传结构,为了解品种间遗传多样性、品种间亲缘关系、育种及科学合理保存和利用现有万寿菊属种质资源提供理论依据和技术支持。【方法】对29份万寿菊材料及2份孔雀草材料利用ISSR分子标记进行遗传关系分析。【结果】用11个引物对31份万寿菊属材料进行PCR扩增,每个引物扩增的ISSR条带数在5—11条,平均每条引物能扩增出6.8条带,多态位点百分率为40%—100%。聚类分析结果表明,ISSR分类结果与传统分类的结果基本一致,31份材料按照万寿菊及孔雀草分为两大类,并且同系列万寿菊品种被划为同一亚组。【结论】利用ISSR标记技术可较准确分析万寿菊属材料间的亲缘关系及遗传多样性。【Objective】 ISSR markers were used to study the genetic relationship and investigate the genetic diversity and genetic structure for providing a theoretical basis and technical support for appropriate conservation and application of existing genetic resources of Tagetes. 【Method】 Genetic relationship of 29 accessions of Tagetes erecta and 2 accessions of T. patula were analyzed using ISSR markers. 【Result】 The genomic DNA of 31 accessions of genus Tagetes was amplified with 11 primers,the bands produced by each primer ranged from 5 to 11,and 6.8 bands were obtained on average,the polymorphic loci ranged from 40%-100%. The cluster analysis of ISSR data indicated that the resources classified by ISSR was basically in accordance with that by traditional method,the accessions were generally classified into 2 groups of different species,and cultivars of same lines were clustered into a subgroup. 【Conclusion】 The genetic relationship and genetic diversity of genus Tagetes could be assessed efficiently by ISSR markers.

关 键 词：万寿菊属 ISSR 品种资源 遗传关系 

分 类 号：S681.9[农业科学—观赏园艺]