玉米Mu转座子及其在反向遗传研究中的应用  

Mutator Transposon in Maize and Its Application in the Reverse genetics

作　　者：崔庆新[1] 李玉玲[1] 杨美丽[1] 冯兴跃 

机构地区：[1]河南农业大学农学院,河南郑州450002 [2]焦作市博爱县农业科学研究所,454450

出　　处：《农业科技通讯》2010年第1期35-38,共4页

基　　金：国家转基因重大专项

摘　　要：Mutator(Mu)是迄今为止发现活性最强的转座子。虽然对其作用机制尚不十分清楚,但已成功用于遗传研究,Mu转座子插入法也已成为克隆玉米基因的主要方法之一。目前B73玉米基因组测序已基本完成,并且在MaizeGDB数据库可以随时查询相关信息,利用反向遗传学方法研究基因的功能更为方便。本文简要介绍了Mu转座子的发现、类型、基本特征,重点介绍了Mu转座子在反向遗传研究中的应用。Mutator （Mu） is the most active and mutagenic plant transposon discovered to date. Although the mechanism of Mu-element regulation was not thoroughly clear, it has been successfully used in the reverse genetics. Mu insertion has become one of the major ways in obtaining mutations and cloning genes in maize. Sequencing of the B73 maize genome is almost finished, and the sequencing data could be accessed in time by MaizeGDB. Using the method of reverse genetics to study gene function will be more convenient. This paper briefly introduced the discovery, types and basic features of Mu transpo son, and focused on its application in reverse genetics.

关 键 词：玉米 Mu转座子 反向遗传学 突变体 

分 类 号：S513[农业科学—作物学]