血管紧张素Ⅱ-NADPH氧化酶-活性氧信号传导通路对人肠系膜动脉平滑肌细胞增殖的影响  

Influence of angiotensin Ⅱ on proliferation of mesenteric artery smooth muscle cells

作　　者：罗廷福[1] 李家富[2] 龚武田[2] 刘全末[2] 王胜利[2] 

机构地区：[1]双流县第二人民医院心血管内科,四川双流610213 [2]泸州医学院附属医院心血管内科,四川泸州646000

出　　处：《西部医学》2010年第1期20-23,共4页Medical Journal of West China

摘　　要：目的观察血管紧张素Ⅱ-NADPH氧化酶-活性氧物质信号传导通路对人肠系膜动脉平滑肌细胞增殖的影响。方法用培养的二至四代人肠系膜动脉平滑肌细胞作为标本;以四甲基偶氮唑盐(MTT)法、流式细胞仪、逆转录聚合酶链式反应(RT-PCR)分别测定细胞增殖率、细胞内活性氧及p22phox蛋白mRNA的表达。结果①加入血管紧张素Ⅱ药物组的细胞增殖率、活性氧物质量、p22phox蛋白mRNA的表达均明显高于空白对照组,差异统计学意义(P<0.05)。②细胞增殖与活性氧物质的生成量、p22phox蛋白mRNA的表达成明显正相关关系,相关系数分别为0.92、0.967。结论血管紧张素Ⅱ在浓度为10-6mol/L时,促进人肠系膜动脉平滑肌增殖、细胞内活性氧物质的生成、细胞内的p22phox蛋白的表达,推测血管紧张素Ⅱ促人肠系膜动脉平滑肌增殖作用部分机制是通过血管紧张素Ⅱ-NADPH氧化酶-活性氧通路来实现。Objective To observe the effect of angiotensin Ⅱ-on NADPH oxidase-active oxygen signal pathway and proliferation in human mesenteric artery smooth muscle cells. Methods Human mesenteric artery smooth muscle ceils were isolated and cultured by organization paste blocks and added angiotensin Ⅱ. Smooth muscle cellular proliferatial rate was determined by MTT assay. The reactive oxygen species was detected with flow cytometry by dihydrochloride acetyl acid dichloride fluorescent yellow （DCFH-DA） as a fluorescent probe of the active oxygen. Cell p22phox mRNA expression changes was tested by reverse transcription polymerase chain reaction （RT-PCR）. Results Compared with the control group, cellular proliferatial rate, fluorescence intensity rate, 22phox mRNA expression of adding angiotensin Ⅱ drug group were significantly increased （P〈0. 05）. Conclusions Angiotensin Ⅱ can promote the mesenteric artery smooth muscle cellular proliferation, formation of reactive oxygen species and p22phox mRNA expression.

关 键 词：血管紧张素Ⅱ NADPH氧化酶 P22PHOX 活性氧 平滑肌细胞 增殖 

分 类 号：R544.1[医药卫生—心血管疾病]