渗透促进剂对荜茇提取物中胡椒碱体外经皮吸收的影响  

Effects of penetration enhancers on the in vitro percutaneous absorption of piperine in Extractum Piperis Longi

作　　者：王丽[1] 郑妮[2] 方亮[2] 余红芳[1] 刘晓秋[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2010年第1期20-23,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的研究荜茇提取物中的主要成分胡椒碱的透皮吸收特性,确定最佳促进剂及质量浓度。方法采用卧式双室扩散池,以离体大鼠皮肤作为渗透屏障,用HPLC法测定样品中胡椒碱的质量浓度。以稳态流量(Js)、增渗比(ER)及滞后时间(tlag)为指标考察渗透促进剂对荜茇提取物中胡椒碱体外经皮吸收的影响。结果当用质量分数为10%的N-甲基-2-吡咯烷酮时,胡椒碱的稳态渗透速率最高。结论N-甲基-2-吡咯烷酮为荜茇提取物中胡椒碱经皮给药有效的渗透促进剂。Objective To investigate the effects of penetration enhancers on the percutaneous absorption of piperine in Extractum Piperis Longi and select the optimal concentration of enhancer.Methods 2-Chamber diffusion cells were adopted as the apparatus for in vitro skin permeation,excised rat abdominal skin as barrier.HPLC method was established to determine the content of piperine in receiver compartment.The steady-state fluxes（Js）and enhancement ratios（ER）were calculated for different penetration enhancers in comparison with control.Results The highest steady-state flux was observed when 10% N-methyl-2-pyrrolidone was added.Conclusions N-methyl-2-pyrrolidone is an effective transdermal delivery enhancers for piperine in Extractum Piperis Longi.

关 键 词：胡椒碱 荜茇提取物 经皮渗透 N-甲基-2-吡咯烷酮 

分 类 号：R94[医药卫生—药剂学]