α_1-酸性糖蛋白柱分离甲溴后马托品溴化物及硫酸阿托品对映体  

Chiral separation of homatropine methylbromide and atropine sulfate using high performance liquid chromatography with α_1-acid glycoprotein column

作　　者：林丽娜[1] 张华燕[1] 郭兴杰[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2010年第1期52-55,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的以α1-酸性糖蛋白(α1-AGP)为固定相,建立甲溴后马托品溴化物及硫酸阿托品的对映体拆分方法。方法采用HPLC法分离。考察流动相中有机改性剂种类和比例、pH值、缓冲盐溶液的浓度、流速、柱温等对对映体分离的影响。结果甲溴后马托品溴化物最佳色谱条件:流动相为10 mmol.L-1醋酸铵(pH值5.5)缓冲液,流速为0.5 mL.min-1,室温;硫酸阿托品最佳色谱条件:流动相为10 mmol.L-1醋酸铵(pH值6.5)缓冲液,流速为0.5 mL.min-1,室温。结论甲溴后马托品溴化物及硫酸阿托品对映体可以在α1-AGP固定相上得到完全分离。Objective To investigate the separation of the enantiomers of homatropine methylbromide and atropine sulfate using high performance liquid chromatographic（HPLC）on α1-acid glycoprotein（α1-AGP）chiral stationary phase.Methods The influence of the pH value and flow rate of the mobile phase,the concentration of buffer solution and organic solvents and temperature were examined.Results The best separation was obtained with 10 mmol·L^-1 NH4OAc（pH 5.5）buffer as mobile phase for homatropine methylbromide and 10 mmol·L^-1 NH4OAc（pH 6.5）buffer as mobile phase for atropine sulfate at ambient temperature.The optimal flow rate was 0.5 mL·min^-1.Conclusions Homatropine methylbromide and atropine sulfate enantiomers can be separated completely on the α1-AGP chiral stationary phase.

关 键 词：α1-酸性糖蛋白固定相 高效液相色谱法 甲溴后马托品溴化物 硫酸阿托品 对映体分离 

分 类 号：R917[医药卫生—药物分析学]