RP-HPLC测定野菊花中槲皮素、木犀草素、芹菜素和刺槐素  

Determination of quercetin,luteolin,apigenin and acacetin in Flos Chrysanthemi Indici by RP-HPLC

作　　者：申海进[1] 郭巧生[1] 房海灵[1] 王艳茹[1] 靳淼[1] 

机构地区：[1]南京农业大学中药材研究所,江苏南京210095

出　　处：《中国中药杂志》2010年第2期191-193,共3页China Journal of Chinese Materia Medica

基　　金：国家科技基础条件平台项目(2005DKA21000)

摘　　要：目的:建立HPLC同时测定野菊花中槲皮素、木犀草素、芹菜素和刺槐素含量的方法。方法:Eclipse XDB-C18柱(4.6 mm×250 mm,5μm);流动相A为甲醇,B为0.2%磷酸溶液,线性梯度洗脱;检测波长350 nm;柱温25℃;流速1.0 mL.min-1;进样量20μL。结果:槲皮素、木犀草素、芹菜素和刺槐素的质量浓度与峰面积分别在1.02～20.48(r=0.999 4),1.03～20.54(r=0.999 2),1.12～22.40(r=0.999 5),1.01～20.22 mg.L-1(r=0.999 8)呈良好的线性关系;平均加样回收率依次为101.3%,100.6%,98.4%,99.02%,RSD为1.3%,1.4%,1.7%,0.83%。不同来源药材间4种被测黄酮组分及总黄酮含量差异均极显著。结论:该方法快速,简便,重复性好,适合于同时测定野菊花样品中槲皮素、木犀草素、芹菜素和刺槐素的含量。Objective： To develop a RP-HPLC method for the determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi indici. Method： An Eclipse XDB-C18 column （4.6 mm×250 mm, 5 μm） was used at 25 ℃ with the mobile phases of methanol-0.2% phosphatic acid in a gradient manner. The flow rate was set at 1.0 mL·min^-1.The detection wavelength was 350 nm. Result： The linear response ranged from 1.02-20.48 mg·L^-1 for quercetin （r=0.999 4, n=5）, 1.03-20.54 mg·L^-1 for luteolin （r=0.999 2,n=5）, 1.12-22.40 mg·L^-1 for apigenin （r=0.999 5, n=5）, 1.01-20.22 mg·L^-1 for acacetin （r=0.999 8, n=5）, respectively. Recoveries were 101.3% with RSD 1.3% for quercetin, 100.62% with RSD 1.4% for luteolin, 98.42% with RSD 1.7% for apigenin and 99.02% with RSD 0.8% for acacetin. A significant difference （α=0.01） among the contents of four flavonoids and total flavonoids was found. Conclusion： The method is quick, simple and repeatable for simultaneous determination of quercetin, luteolin, apigenin and acacetin in Flos Chrysanthemi Indici.

关 键 词：野菊花 槲皮素 木犀草素 芹菜素 刺槐素 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]