四妙勇安汤对动脉粥样硬化模型兔氧化应激及炎症反应的影响  

Effect of Simiao Yong'an Decoction on Oxidative Stress and Inflammation on Atherosclerosis Model Rabbits

作　　者：张军平[1] 许颖智[2] 李明[1] 李良军[2] 彭立[2] 张光银 杨萃[2] 周亚男[2] 

机构地区：[1]天津中医药大学第一附属医院天津市南开区鞍山西道314号,300193 [2]天津中医药大学

出　　处：《中医杂志》2010年第1期72-74,共3页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30672734)

摘　　要：目的探讨四妙勇安汤对动脉粥样硬化家兔的干预效果及作用机理。方法36只日本雄性大耳白兔随机分为4组,分别为正常对照组、模型组、四妙勇安组及辛伐他汀组。通过高脂饲料、免疫损伤结合经股动脉球囊拉伤建立动脉粥样硬化家兔模型,四妙勇安组给予四妙勇安汤每次11.25g/kg生药量灌胃,每日2次;辛伐他汀组给予辛伐他汀5mg/kg灌胃,每日1次;正常对照组和模型组每日灌胃生理盐水20ml。于0、3、6、10周时测定各组家兔血超氧化物歧化酶(SOD)、丙二醛(MDA)、白细胞介素-1(IL-1)、肿瘤坏死因子α(TNF-α)、单核细胞趋化因子(MCP-1)的含量及主动脉细胞核因子-κB(NF-κB)的表达。结果与正常对照组比较,模型组各时间点MDA、IL-1、TNF-α、MCP-1水平显著增高(P<0.05或P<0.01),SOD与TNF-α之间存在一定相关性,四妙勇安组和辛伐他汀组在各时间点能够不同程度的调节MDA、IL-1、MCP-1、TNF-α、MCP-1水平及动脉壁NF-κB表达(P<0.05或P<0.01)。结论四妙勇安汤能够抑制动脉粥样硬化家兔炎症反应及氧化应激,增强兔动脉粥样硬化斑块的稳定性。Objective To observe the effect of Simiao Yong＇an Decoction （Decoction of four herbs for atherosclerosis） on rabbit atherosclerosis and its mechanism. Methods Thirty-six Japanese white rabbits were randomized into four groups： normal control group, model group, Simiao Yong＇an group and simvastain group. The models were made through high fat diet, immune injury combined with femoral balloon bulling injury. Simiao Yong＇an group was treated by Simiao Yong＇an Decoction, raw medicine 11.25g/kg by gavage each time, twice a day; Simvastain group was treated by simvastain 5mg/kg by gavage each time, once a day;normal control group and model group were treated by normal saline by gavage, 20ml each day. In the 0, 3rd , 6th , and 10th week, the concentration of superoxide dismutase （SOD）, malondialdehyde （MDA）, interleukin （IL-1）, tumor necrosis factor-α （TNF-α）, monoeyte ehemoattractan protein-1 （MCP-1）, and the expression of aortic nucleus factor-κB （NF-κB） were determined. Results Compared with those of the normal control group, the level of MDA, IL-1 ,TNF-α, MCP-1, and NF-κB of model group increased significantly at all time points （P〈0.05 or P〈0.01）, and there was relativity between SOD and TNF-α. Simiao Yong＇an group and simvastain group could regulate MDA, IL-1, MCP-1, TNF-α, and MCP-1 at all tome points to different degrees and the expression of NF-κB in aorta wall （P〈0. 05 or P〈0. 01）. Conclusion Simiao Yong＇an Decoction can prohibit inflammation reaction and oxidative stress of atherosclerosis rabbits, strengthening the stability of atherosclerosis plague.

关 键 词：四妙勇安汤 动脉粥样硬化 氧化应激 炎症反应 

分 类 号：R285.5[医药卫生—中药学] R289.5[医药卫生—中医学]