活血化瘀消癥通络中药对糖尿病肾病大鼠血管紧张素系统的影响  

Effect of Blood-activating and Stasis-dissolving Herbs on Renin Angiotensin System in Diabetic Nephropathy Rats

作　　者：郭登洲[1] 王月华[1] 边东 刘晓冉[1] 陈志强[1] 

机构地区：[1]河北医科大学中西医结合研究所石家庄市中山东路361号,050017

出　　处：《中医杂志》2010年第1期75-78,共4页Journal of Traditional Chinese Medicine

基　　金：河北省科学技术研究与发展计划项目(07276101D-111,06276102D-23);河北省自然科学基金资助项目(C2008001074)

摘　　要：目的观察活血化瘀消癥通络中药对糖尿病肾病(DN)大鼠作用及其机制。方法将46只健康雄性SD大鼠随机分为正常组(10只),另36只一次性腹腔注射链脲佐菌素制备DN大鼠模型,随机分为模型组、贝那普利组、中药组,每组各12只。模型制备成功1周后贝那普利组、中药组每日同体积灌胃给予相应药物,正常组及模型组灌喂等量蒸馏水,各组连续给药23周后处死动物。HE、PAS染色观察肾组织病变程度,放射免疫法测定大鼠肾组织中血管紧张素(Ang)的含量、采用紫外分光光度法测定血管紧张素转换酶(ACE)活性,免疫组织化学法和RT-PCR法检测大鼠肾组织中ACE、血管紧张素转换酶相关羧肽酶(ACE2)蛋白和基因的表达。结果与模型组相比,中药组大鼠肾小球毛细血管基底膜增厚及肾小球系膜细胞增生、系膜基质增多、系膜区增宽等现象明显减轻;中药组大鼠肾组织中Ang的含量和ACE活性较模型组降低(P<0.01),肾组织中ACE2蛋白及基因的表达显著增高(P<0.05或P<0.01)。结论活血化瘀消癥通络中药能够减轻DN的病理损害,这一作用的实现可能是部分通过调节血管紧张素系统实现的。Objective To observe the effect of blood-activating and stasis-dissolving herbs on rats with diabetic nephropathy （DN） and its mechanism. Methods Forty-six healthy male Sprague-Dauley rats were randomized into normal group （10 rats） and DN model group （36 rats） was established by streptozotocin injection into abdominal cavity, which was subrandominzed into model group, benazepril group, and herbal group, 12 rats in each. One week after modelling, the treatment groups were medicated by garage in the same volume, and the normal and model groups were given distilled water of the same volume in succession for 23 weeks, and then killed. HE and PAS staining was done to observe the pathological changes of renal tissues, radioimmunoassay was used to determine the level of angiotensin Ⅱ （Ang Ⅱ）, ultraviolet spectrophotometry was applied to measure the activity of angiotensin conversion enzyme （ACE）, and immunohistochemistry was employed to test the expressions of ACE and ACE-related carboxypeptidase （ACE2） in the renal tissues of rats. Results Compared with the model group, the thickened glomerular basement membrane, increased mesangial cells, proliferated mesangial matrix, and enlarged mesangial area became lightened. In addition, herbal treatment could decrease the level of Ang 71 and active ACE （P〈0. 05） and increase the expression of ACE2 （P〈0. 05）. Conclusion The blood-activating and stasis-dissolving herbs have the funetion to reduce the pathological damage of DN, possibly partially through the regulation of renin angiotensin system.

关 键 词：活血化瘀消瘕通络 血管紧张素Ⅱ 血管紧张素转换酶 血管紧张素转换酶相关羧肽酶 糖尿病肾病 

分 类 号：R285.5[医药卫生—中药学]