抗肿瘤中药青龙衣化学成分的研究  

Studies on constituents from pericarps of Juglans mandshurica with anti-tumor activity

作　　者：周媛媛[1] 王栋[1] 牛峰 

机构地区：[1]黑龙江中医药大学药学院,黑龙江哈尔滨150040 [2]哈药集团制药六厂,黑龙江哈尔滨150056

出　　处：《中草药》2010年第1期11-14,共4页Chinese Traditional and Herbal Drugs

基　　金：黑龙江省研究生创新科研项目(YJSCX2007-0177HLJ)

摘　　要：目的研究抗肿瘤中药青龙衣的化学成分。方法近年大量的药理实验及临床研究表明青龙衣CHCl3及EtOAc部分具有显著的抗肿瘤作用，且毒性较小。本实验利用硅胶柱色谱和大孔吸附树脂分离化合物，并利用1D-NMR及2D-NMR光谱数据确定了化合物的结构。结果从青龙衣的CHCl3活性部位中分得8个化合物，并分别确定为20（S）-原人参二醇-3-酮（1）、达玛烷-20，24-二烯-3β-醇（2）、茸毛香杨梅酮（galeon）（3）、胡桃宁A（juglanin A）（4）、2a，3β，23-三羟基-12-烯-28-齐墩果酸（5）、2a，3β—23-三羟基-12-烯-28-熊果酸（6）、齐墩果酸（7）、熊果酸（8）。结论化合物1为首次从天然资源中分离得到的新天然产物；化合物2及5～8均为首次从胡桃属植物中分离得到，此前未见该属三萜类化合物的报道；化合物3为首次从青龙衣中分离得到。Objective To study the chemical components from the pericarps of Juglans mandshurica with anti-tumor activity. Methods It showed that pericarps of J. mandshurica had a good performance in treating cancer and low toxicity from CHC13 and EtOAc portions by a lot of pharmacological experiments and modern clinical application in the past. Compounds were isolated by chromatography on silica gel columns and AB-8 macroporous resin. On the basis of spectral evidences including 1D-NMR and 2D-NMR,the structures of these compounds were determined. Results These compounds from CHC13 portion were identified as 20（S）-protopanaxadiol-3-one （1）, dammara-20, 24-dien-317-ol （2）, galeon （3）, juglanin A （4）, 2a, 3β, 23-trihydroxy olean-12-en-28-oic acid （5）, 2α, 3β, 23-trihydroxy urs-12-en-28-oic acid （6）, oleanolic acid （7）, ursolic acid （8）. Conclusion Compound 1 is a new natural product. Compounds 2 and 5-8 are isolated firstly from the plants of Juglans L. which are due to pentacyclic triterpane. Compound 3 is isolated from the pericarps of J. mandshurica for the first time.

关 键 词：青龙衣 20(S)-原人参二醇-3-酮 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]