双氢青蒿素诱导前列腺癌PC-3细胞凋亡及其机制研究  

Induction of dihydroartemisinin on prostate cancer PC-3 apoptosis and its mechanism

作　　者：高小玲[1] 罗子国[1] 王丕龙[2] 李庆春[1] 

机构地区：[1]重庆医科大学生命科学研究院,重庆400016 [2]重庆医科大学附属第一医院消化内科,重庆400016

出　　处：《中草药》2010年第1期81-85,共5页Chinese Traditional and Herbal Drugs

摘　　要：目的研究双氢青蒿素对前列腺癌PC-3细胞的凋亡诱导作用,并探讨其可能的机制。方法MTT法测定不同浓度双氢青蒿素对PC-3细胞增殖的影响,流式细胞仪(FCM)、透射电镜(TEM)观察双氢青蒿素对PC-3细胞的凋亡诱导作用,免疫组化(SP)检测双氢青蒿素对PC-3细胞内Bax、Bcl-2蛋白阳性表达的影响。结果双氢青蒿素对PC-3细胞有明显增殖抑制效应,能诱导PC-3细胞凋亡且具有浓度-时间依赖性,导致PC-3细胞线粒体肿胀、核碎裂、凋亡小体形成;随着双氢青蒿素浓度的增加,Bcl-2蛋白阳性表达降低,而Bax蛋白阳性表达增加。结论双氢青蒿素可能通过上调Bax,下调Bcl-2蛋白表达诱导前列腺癌PC-3细胞凋亡。Objective To study the induction of dihydroartemisinin （DHA） on prostate cancer PC-3 apoptosis and its possible mechansim. Methods MTT was employed for cellular viability measurement, flow cytometry （FCM） and transmission electron microscopy （TEM） for observation of apoptosis, and immunocytochemical staining （SP） for analyzing the expression of Bcl-2 and Bax proteins in PC-3 cells treated with DHA of different concentration. Results DHA Significantly inhibited the proliferation of PC-3 cells, induced their apotosis in a time-concentration dependent manner, and led to mitochondrial swelling, nuclear fragmentations and apoptosis body formation, down-expression of Bcl-2 protein, and over-expression of Bax protein correspondence with DHA concentration. Conclusion DHA could induce the apoptosis in PC-3 cells by up-regulating Bax protein and down-regulating Bcl-2 protein.

关 键 词：双氢青蒿素 前列腺癌 细胞凋亡 

分 类 号：R979.1[医药卫生—药品] R737.2[医药卫生—药学]