明党参内生真菌种群的生态分布及其诱导子活性研究  

Ecological distribution and elicitor activities of endophytic fungi in Changium smyrnioides

作　　者：江曙[1] 段金廒[1] 陶金华[1] 严辉[1] 郑建兵[1] 

机构地区：[1]南京中医药大学,江苏南京210046

出　　处：《中草药》2010年第1期121-125,共5页Chinese Traditional and Herbal Drugs

基　　金：江苏省自然科学基金(BK2007241);南京中医药大学青年科学基金(09XZR19);江苏省方剂研究重点实验室资助项目

摘　　要：目的研究不同产区、不同生长期明党参内生真菌的种群结构与生态分布,以及内生真菌诱导子对明党参细胞生长与多糖积累的影响。方法采用微生物分离、培养与鉴定等生物学方法、植物细胞悬浮培养方法以及统计分析方法。结果从4个产地明党参植株中共分离到8属116株内生真菌,其中镰孢属(Fusarium LK.exFR.)、地霉属(Geotrichum LK.)和链格孢属(Alternaria Nees)为优势种群。随着明党参生长期的不同,内生真菌种群结构出现明显的动态变化。在苗期和孕蕾期,内生真菌种群丰富,数量较多,尤其是在孕蕾期3种优势种群的分离率和分离频率分别达到30%和19%以上。部分内生真菌种群还具有明显的区域专一性和组织专一性。此外,内生真菌Fusarium sp.3诱导子处理组,明党参细胞生长量和多糖量分别比对照提高了31.86%和38.01%。结论明党参内生真菌具有丰富的生物多样性,种群结构及分布与其生态环境具有密切的相关性,且部分明党参内生真菌诱导子对其细胞生长和多糖积累均具有显著的促进作用。Objective The population structure and ecological distribution of endophyt Changium smyrnioides from the different habitats and growing phases, and the effects of funga the cell biomass and polysaccharide accumulation were studied in this paper. Methods The iso c fungi in elicitor on ation, culture, and identification of microorganism, and plant cell suspension culture technology were adopted; And relative data were analyzed by the statistical methods. Results In four producing areas, 116 strains were isolated and classified into eight genera. The dominant populations were Fusarium LK. ex FR. , Geotrichum LK. , and Alternaria Nees. The population structure of endophytic fungi obviously changed at the different growing phases. Species and quantity of endophytic fungi were plentiful at the seedling stage and bud stage, and especially at the bud stage the isolation rate and isolation frequency were more than 30% and 19%, respectively. Some endophytic fungi had the obvious area and tissue specificity. Compared with the control by adding the elicitor of Fusarium sp. 3, the yields of cell biomass and polysaccharide were increased to 31.86% and 38.01%, respectively. Conclusion Endophytic fungi in C. smyrnioides have abundant biodiversity. And there is close relationship between the population structure and distribution of endophytic fungi with ecological conditions. And fungal elicitors could obviously enhance the yields of cell biomass and polysaccharide of C. smyrnioides.

关 键 词：明党参 内生真菌 生态分布 诱导子 

分 类 号：R282.15[医药卫生—中药学]