注意缺陷多动障碍大鼠前额叶中Homer家族表达研究  

Expression of Homer in prefrontal cortex of rats with attention-deficit hyperactivity disorder

作　　者：洪琴[1] 池霞[1,2] 郭锡熔[1,2] 童梅玲[1] 张敏[1] 陈荣华[2] 费莉[2] 郭梅[2] 潘晓勤[2] 

机构地区：[1]南京医科大学附属南京市妇幼保健院儿保科,南京210004 [2]南京医科大学儿科医学研究所,南京210029

出　　处：《中国实用儿科杂志》2010年第1期64-67,共4页Chinese Journal of Practical Pediatrics

基　　金：国家自然科学基金(30801255);江苏省自然科学基金(BK2006009)

摘　　要：目的探讨Homer家族在注意缺陷多动障碍(ADHD)动物模型幼年自发性高血压(SHR)大鼠与Wistar京都(WKY)大鼠前额叶中的表达差异情况,分析其与ADHD的关系。方法2006年12月至2007年7月在南京医科大学儿科医学研究所应用反转录-聚合酶链反应(RT-PCR)、免疫蛋白印迹技术(Western Blotting)检测SHR与WKY大鼠前额叶皮质中Homer1a、Homer1b/c、Homer2a/bmRNA和蛋白的表达情况。结果RT-PCR结果显示,SHR大鼠Homer1a、Homer2a/bmRNA表达量较WKY大鼠降低,差异有统计学意义(t=2.56,P<0.05;t=5.85,P<0.05),Homer1b/c在两组间差异无统计学意义(t=0.44,P>0.05)。Western Blotting结果显示,SHR大鼠Homer1a、Homer2a/b蛋白表达量较WKY大鼠降低,差异有统计学意义(t=2.76,P<0.05;t=3.40,P<0.05)。结论SHR大鼠前额叶皮质Homer1a、Homer2a/b表达较WKY大鼠显著降低,提示Homer1a、Homer2a/b可能与ADHD密切相关。Objective To investigate the expression of Homer on Attention-Deficit Hyperactivity Disorder （ADHD）. Methods The total RNA and protein were extracted from prefrontal cortex （PFC） of SHR and WKY rats. Both RT-PCR technique and Western Blotting technique were used to detect the expression levels of different isoforms of Homer （Homer 1a, Homer 1b/c, Homer 2a/b） in PFC of SHR and WKY rats. Results （1） By RT-PCR detection, it was found that the expression levels of Homer la and Homer 2a/b gene were significantly lower in prefrontal cortex of SHR rats than those in WKY ones （t = 2.56,P 〈 0.05;t = 5.85,P 〈 0.05）. There was no difference in the expression levels of Homer 1b/c gene in prefrontal cortex between SHR and WKY rats （t = 0.44, P 〉 0.05）. （2） By Western Blotting, it was found that the expression levels of Homer la and Homer 2a/b gene were significantly lower in prefrontal cortex and hippocampus of SHR rats than those in WKY rats （ t = 2.76, P 〈 0.05 ; t = 3.40, P 〈 0.05）. Conclusion Homer la and Homer 2a/b may play an important role in pathogenesis of ADHD.

关 键 词：注意缺陷多动障碍 前额叶皮质 反转录-聚合酶链反应 免疫蛋白印迹技术 

分 类 号：R72[医药卫生—儿科]