卷柏穗花杉双黄酮的舒张血管作用实验研究  

Experiment study on vasodilative effects of amentoflavone ethyl acetate extract of selaginella tamariscina

作　　者：许兰[1] 尹明浩[1] 

机构地区：[1]延边大学中医学院,吉林延吉133000

出　　处：《延边大学医学学报》2009年第4期246-248,共3页Journal of Medical Science Yanbian University

摘　　要：[目的]探讨卷柏乙酸乙酯提取物穗花杉双黄酮对血管紧张性的影响及其可能的机制.[方法]利用多道生理记录仪对大鼠离体主动脉环进行观察,了解卷柏己烷、乙酸乙酯和正丁醇提取物对血管的作用,并从血管内皮细胞、一氧化氮和受体角度探讨其机制.[结果]卷柏的3种提取液均有明显的舒张血管作用,其中以乙酸乙酯提取物穗花杉双黄酮的作用最强,且在0.1～10.0μmol/L范围内具有剂量依赖性.损伤大鼠胸主动脉环内皮后,穗花杉双黄酮的舒张血管作用被抑制;在内皮完整的动脉血管环使用一氧化氮抑制剂L-NAME后,穗花杉双黄酮的作用亦被抑制;使用1.0μmol/L盐酸普萘洛尔或阿托品对穗花杉双黄酮的舒张血管作用无影响.[结论]卷柏乙酸乙酯提取物穗花杉双黄酮的舒张血管作用存在内皮依赖性,且有一氧化氮参与.OBJECTIVE To investigate the effects of ethyl acetate extract of the selaginella tamariscina （ST） on vasotonia and its underlying mechanisms. METHODS Using the polygraph,the effects of hexane,ethyl acetate and n-bulanol extracts of the ST for the vasotonia were evaluated with the model of isolated rat thoracic aorta rings in organ bath,and the mechanisms were studied by the point of vascular endothelial cell,nitric oxide （NO） and receptor. RESULTS All of three extracts of the ST had the effects of vasodilation,in which the ethyl acetate extract of the ST had the strongest effects with the dose-dependent at the concentrations of 0.1～10.0 μmol/L. But this effect was almost inhibited after removal of rat thoracic aorta rings and pretreating the endothelium intact aorta rings with NG nitro-L-arginine methyl ester at the concentration of 1.0 μmol/L,respectively. Thepropranolol （1.0 μmol/L） or atropine （1.0 μmol/L） did not affect vasodilation with ethyl acetate extract of the ST. CONCLUSION The ethyl acetate extract of the ST has the effect of vasodilation with endothelium dependent and engaging in NO.

关 键 词：穗花杉双黄酮 主动脉 血管舒张 一氧化氮 大鼠 

分 类 号：R285.5[医药卫生—中药学]