甲胎蛋白干血滤纸免疫放射分析试剂盒的研制  

DEVELOPMENT OF AFP BLOOD SPOT IMMUNORADIOMETRIC ASSAY

作　　者：陈键[1] 刘一兵[1] 韩世泉[1] 王衍真[1] 

机构地区：[1]中国原子能科学研究院同位素研究所

出　　处：《同位素》1998年第3期134-138,共5页Journal of Isotopes

摘　　要：用氯胺Ｔ法制备１２５Ｉ－ＡＦＰＭｃＡｂ，用ＡＦＰＭｃＡｂ包被试管，以全血作标准，建立了ＡＦＰ干血滤纸片免疫放射分析方法。该方法灵敏度为７．２μｇ／Ｌ，批内变异系数为５．１％—１０．０％，批间变异系数为４．７％—８．６％，正常参考值≤１０μｇ／Ｌ，与血清ＡＦＰ免放分析方法比较，相关系数ｒ＝０．Two AFP McAb are used in AFP blood spot immunoradiometric assay. One is labeled with iodine 125 and the other is coated to the test tubes. The whole blood standards are spotted on filter paper. The measurement range is 10-800 μg/L. The sensitivity is no more than 7.2 μg/L. The intra and inter assay coefficient of variation are 5.1%-10.0% and 4.7%-8.6% respectively. The normal value is less than 10 μg/L. The corretation coefficient comparing with the IRMA of serum AFP is 0.979.

关 键 词：甲胎蛋白 干血滤纸 免疫放射分析 试剂盒 

分 类 号：R392－33[医药卫生—免疫学] R817[医药卫生—基础医学]