高纯度^(125)I-蛇毒神经生长因子的制备  

PREPARATION OF HIGHLY PURIFIED 125 I VENOM NEURAL GROWTH FACTOR

作　　者：刘秀文[1] 汤仲明[1] 柴彪新[1] 汪家政[2] 

机构地区：[1]军事医学科学院放射医学研究所 [2]军事医学科学院基础医学研究所

出　　处：《同位素》1998年第3期144-148,共5页Journal of Isotopes

摘　　要：介绍了１２５Ｉ－蛇毒神经生长因子（ＮＧＦ）的制备及鉴定。标记前纯品在ＷａｔｅｒｓＳＰ磺酸型阳离子交换高效液相（ＨＰＩＥＣ）中表现为单一峰，但１２５Ｉ标记后Ｓｅｐｈａｃｒｙ１Ｓ－２００ＨＲ凝胶标记蛋白部分的ＨＰＩＥＣ谱，除１２５Ｉ－蛇毒ＮＧＦ外，出现了分子量较大、与凝胶柱不结合的未知组分，提示可能发生聚合和表面电荷变化。结合蛇毒ＮＧＦ性质选用Ｓｅｐｈａｒｏｓｅ－ＳＰ阳离子交换柱去除上述未知组分。放化纯度和生物鉴定证明：两步纯化标记产物得到了放化纯度为９７％、有生物活性的１２５Ｉ－蛇毒ＮＧＦ。提示根据多肽特点选择纯化和鉴定方法是制备高纯度有活性标记多肽的重要环节。The highly purified and bio active 125 I vNGF is prepared. The high performance ionexchange chromatographic(HPIEC) profile of non labeled vNGF is appeared as a single peak eluted at about 0.4 mol/L -1 NaCl. After 125 I labeling and purification with Saphacryl HR200, the HPIEC profile of labeled protein exhibits a new large molecule that is not absorbed on the cation column. The result suggests that there may be aggregation and surface electronic changes during the labeling. After the second separation using Sepharose SP to remove the non absorbed component, a 97% 125 I vNGF is obtained. The bioassay of 125 I vNGF demonstrates the good biological activity. The result suggests that it is important to design an optimal purification method, according to the physical chemistry property of the labeled protein.

关 键 词：蛇毒 神经生长因子 生物活性检定 碘125标记 

分 类 号：R817[医药卫生—影像医学与核医学] R996.3[医药卫生—放射医学]