柯萨奇B_3病毒对小鼠离体心肌细胞力学和收缩蛋白分子的影响  

The cardiac myocyte mechanics and gene expression of myocardial contractile protein in murine myocytes infected with coxsackievirus B

作　　者：万朝敏[1] 彭文珍[1] 高云钦 王德诚 刘惠莲[1] 王正荣 

机构地区：[1]华西医科大学附属第二医院儿科

出　　处：《中华儿科杂志》1998年第9期531-533,共3页Chinese Journal of Pediatrics

基　　金：国家自然科学基金

摘　　要：目的探讨病毒性心肌炎时病毒直接损伤心肌细胞的发病机理。方法培养新生ＢＡＬＢ／ｃ小鼠的离体心肌细胞，加柯萨奇Ｂ３病毒，造成病毒对心肌细胞直接损伤的细胞模型，检测心肌细胞力学和心肌收缩蛋白分子———心肌肌凝蛋白的α重链（αＭＨＣ），β重链（βＭＨＣ）的基因表达。结果柯萨奇Ｂ３病毒对体外培养的心肌细胞有高度的易感性，并造成收缩力下降，其中感染２４小时的心肌细胞收缩力减弱比１２小时更为明显；感染后的心肌细胞收缩蛋白分子发生了改变，表现为αＭＨＣ含量降低（由０．１５±０．０２减少到０．１３±０．０６），而βＭＨＣ含量增加（由０．０８７±０．００８增加到０．１１１±０．００９）。结论病毒直接损伤心肌细胞，使心肌细胞收缩蛋白分子的结构发生了改变。Objective To determine the mechanism responsible for direct damage of cardiac muscle cell by virus in myocarditis. Methods Cellular model of directly injuried cardiac muscle cell by Coxsackievirus B3(CVB3) was established by adding CVB3 into the culture of cardiac muscle cells to detect cardiac muscle cell mechanics and the genes expression of myocardial contractile protein, αmyosin heavy chain (αMHC) and βMHC. Results Cultured cardiac muscle cell was susceptible to CVB3. The contractility of cardiac muscle cell infected by CVB3 decreased significantly as compared with normal cardiac muscle cell and decreased more significantly at 24 hours than at 12 hours after infection by CVB3. The gene expression of αMHC mRNA by cardiac muscle cell infected by CVB3 was significantly decreased (from 0.15±0.02 to 0.13±0.06) but that of βMHC mRNA was increased (from 0.087±0.008 to 0.111±0.009) as compared with normal cardiac muscle cells. Conclusion CVB3 may directly damage cardiac muscle cell, alter the structure of myocyte contractile proteins and therefore reduce the cardiac muscle cell contractility.

关 键 词：柯萨奇病毒B 收缩蛋白 病毒性心肌炎 心肌力学 

分 类 号：R725.422.1[医药卫生—儿科]