哮喘患者外周血单个核细胞中白细胞介素-6 mRNA表达与气道炎症的研究  

Research of interleukin 6 mRNA gene expressions in peripheral blood mononuclear cells and airway inflammation in allergic asthmatic patients

作　　者：陈萍[1] 张齐武[1] 刘民培[1] 林小平[1] 李春涛[1] 康林[1] 董涓[1] 

机构地区：[1]沈阳军区总医院呼吸内科

出　　处：《中华内科杂志》1998年第9期583-585,共3页Chinese Journal of Internal Medicine

摘　　要：目的探讨外周血单个核细胞（ＰＢＭＣ）中白细胞介素（ＩＬ）６ｍＲＮＡ表达与血嗜酸细胞阳离子蛋白（ＥＣＰ）和１秒用力呼气容积占用力肺活量比值（ＦＥＶ１％）的关系。方法对１２例过敏性哮喘发作期患者、８例缓解期患者及９例健康人，采用ＲＴＰＣＲ法和图像分析半定量法检测ＰＢＭＣ中ＩＬ６ｍＲＮＡ的表达水平及ＥＣＰ和ＦＥＶ１％。结果发作期患者ＩＬ６ｍＲＮＡ的表达明显高于缓解期患者和健康人（Ｐ＜００１），而缓解期患者无明显升高。发作期患者ＩＬ６ｍＲＮＡ的表达水平与血清ＥＣＰ呈中度正相关（ｒ＝０６７９，Ｐ＜００１），而与ＦＥＶ１％呈中度负相关（ｒ＝－０５８９，Ｐ＜００５）。结论过敏性哮喘发作期患者ＰＢＭＣ中ＩＬ６ｍＲＮＡ表达水平显著增强。Objective To study the interrelation among interleukin (IL) 6 mRNA gene expression in peripheral blood mononuclear cells (PBMC), eosinophil cation protein (ECP) and forced expiratory volume in one second to forced vital capacity ratio (FEV 1%). Methods RT PCR method and image pattern analysis semiquantitation method were used to determine IL 6 mRNA gene expression level in PBMC, ECP and FEV 1% in 12 allergic asthmatic patients during acute attack, 8 allergic asthmatic patients in remission and 9 normal subjects. Results IL 6 mRNA in acute attack group was 1.5789±0.8732, being higher than that in remission group (0.2200± 0.0474) and normal group (0.1667±0.0689, P <0.01), while IL 6 mRNA in remission group was not elevated significantly ( P >0.05). IL 6 mRNA expression level in allergic asthmatic patients were moderately positive rela ted with serum ECP ( r =0.679, P <0.01) and moderately negative related with FEV 1% ( r =-0 589, P < 0.05). Conclusion IL 6 mRNA expression level in PBMC during acute attack of allergic asthma was significantly augmented and eosinophils activation increased. The degree of airway obstruction was related to the increased transcription of IL 6 genes.

关 键 词：哮喘 单核细胞 白细胞介素-6 mRNA 气道炎症 

分 类 号：R562.250.3[医药卫生—呼吸系统]