溴酚蓝光度法测定壳聚糖的研究  

Spectrophotometric Determination of Chitosan with Bromophenol Blue

作　　者：马卫兴[1] 朱鹏[1] 王敏[1] 周亚文[1] 

机构地区：[1]淮海工学院化学工程学院,江苏连云港222005

出　　处：《食品与发酵工业》2009年第12期127-129,共3页Food and Fermentation Industries

基　　金：江苏省高校自然科学研究计划资助项目(No.05KJB150003);全国化工高等教育科学研究"十一五"规划立项课题(No.中化教协发[2007]003-33);江苏省海洋生物技术重点建设实验室资助项目(2005HS010);江苏省2007年大学生创新计划项目

摘　　要：在pH=2.87 Britton-Robinson缓冲介质中基于溴酚蓝与壳聚糖发生相互作用形成在630nm处有最大吸收的绿色复合物,建立了壳聚糖的测定新方法。通过实验对该结合反应的条件进行优化,壳聚糖在1.0-12.5μg/mL内服从比尔定律,其工作曲线的回归方程为A=0.003301c-0.0021,相关系数R=0.9990。所拟方法用于胶囊中壳聚糖的测定,结果与溴甲酚绿法所得结果一致。The content of chitosan was determined by the absorption of the green complex formed by reaction of bromophenol blue with chitosan in Britton-Robinson buffer medium. The optimum conditions for determination of the chitosan were investigated. Beer＇ s law was obeyed in the concentration range of 10 - 125μg ehitosan per 10 mL with the regressive equation ofA = 0. 003 301 c（μg/10mL） -0. 002 1 and the coefficient R of 0. 999 0. The proposed method was applied to determine the content of chitosan in capsules with the same results with that of bromocreso green method.

关 键 词：壳聚糖 溴酚蓝 光度法 

分 类 号：O636.1[理学—高分子化学] TQ153.2[理学—化学]