超高效液相色谱-电喷雾串联四级杆质谱法快速分析水中微囊藻毒素LR  

Rapid Determination of Microcystin-LR in Water by UPLC-ESI-MS/MS

作　　者：茅海琼[1] 翁燕波[1] 傅晓钦[1] 朱丽波[1] 胡迪峰[1] 

机构地区：[1]宁波市环境监测中心,浙江宁波315012

出　　处：《中国环境监测》2009年第6期19-22,共4页Environmental Monitoring in China

摘　　要：建立超高效液相色谱-电喷雾串联四极杆质谱快速测定水中微囊藻毒素LR（MC-LR）的方法。水样经0.2μmGHP一次性针头过滤器过滤,应用超高效液相色谱/电喷雾串联四极杆质谱仪多离子反应监测（MRM）法定量检测MC-LR。经方法学验证,该方法对MC-LR的最低检出限LOD为0.08μg/L（进样量10μl）,最低定量限LOQ是0.10μg/L。在0.2-20.0μg/L的线性范围中,相关系数r=0.9982,回收率范围91.5%-110.3%。方法灵敏度高,专属性强,操作简便快速,定量准确,测定浓度范围宽,是环境水质样品中MC-LR含量检测的理想方法。Objective： A UPLC-ESI-MS/MS method was developed for the determination of microcystin-LR in water.Methods： 0.2μm membrane filter was used to remove dirties.UPLC analysis was performed on a Waters ACQU ITY UPLC BEH C18 2.1×50mm （1.7μm） column,acetonitrile-0.3% acetic acid solution（50∶50）was used as mobile phase.microcystin-LR was determined by UPLC-ESI-MS/MS and quantified with multiple reaction monitoring（MRM） mode.Results： The detection limit （LOD） for microcystin-LR was 0.08μg/L.The limit of quantification（LOQ） was 0.10μg/L.The linear range was 0.2～20.0μg/L with correlation coefficient 0.9982.The mean recoveries were between 91.5% and 110.3%.Conclusion： This method can be applied for the determination of microcystin-LR in water for its high sensitivity,high selectivity and simple pre-process.

关 键 词：超高效液相色谱-电喷雾串联四极杆质谱 多离子反应监测 微囊藻毒素LR 

分 类 号：X832[环境科学与工程—环境工程]