钙拮抗剂TMB-8对培养牛大脑中动脉内皮细胞[Ca^(2+)]i和一氧化氮释放的影响  

EFFECT OF 8-(N,N′-DIETHYLAMINO)-n-OCTYL-3,4,5-TRIMETHOXYBENZOATE ON [Ca^(2+)]i AND THE RELEASE OF NO IN CULTURED ENDOTHELIAL CELLS OF THE CALF MIDDLE CEREBRAL ARTERY

作　　者：王斌 俞惠兰 

出　　处：《药学学报》1998年第9期650-654,共5页Acta Pharmaceutica Sinica

摘　　要：目的旨在观察ＴＭＢ８对血管内皮细胞［Ｃａ２＋］ｉ水平和ＮＯ释放的影响，探讨扩张脑血管的机制。用ＡＲＣＭＭＩＣ阳离子测定系统，测量单个细胞内游离钙浓度（［Ｃａ２＋］ｉ），用血红蛋白法测量一氧化氮（ＮＯ）的释放。结果表明，在细胞外钙浓度为１３ｍｍｏｌ·Ｌ－１时，ＴＭＢ８１２５及２５０μｍｏｌ·Ｌ－１对静息［Ｃａ２＋］ｉ和甲基血红蛋白ΔＥ无明显影响，而５０及１００μｍｏｌ·Ｌ－１时可升高静息［Ｃａ２＋］ｉ和甲基血红蛋白ΔＥ。表明ＴＭＢ８５０及１００μｍｏｌ·Ｌ－１升高脑血管内皮［Ｃａ２＋］ｉ，激活ＮＯ合酶，促进ＮＯ合成和释放，这可能是其扩张脑血管的重要机制之一。The effect of 8-(N,N′-diethylamino)-n-octyl-3,4,5-trimethoxybenzoate(TMB-8) on [Ca^(2+)]i and the release of NO in cultured endothelial cells of the calf middle cerebral artery was studied by a system of measurement of AR-CM-MIC, using Fura2/AM as a fluoresent indicator and the oxyhaemoglobin method. In the presence of extracellular Ca^(2+) 13 mmol·L-1, the resting [Ca^(2+)]i and the extinction difference(ΔE) of between 401 and 410 nm were not changed by TMB8 125 and 25 μmol·L-1, but were increased by TMB8 50 and 100 μmol·L-1. The rise of ΔE induced by TMB8 50 and 100 μmol·L-1 was blocked completely by LNAME. The elevation of [Ca^(2+)]i was related to the release of NO. The result suggested that TMB8 increased resting [Ca^(2+)]i of endothelial cells of the calf middle cerebral artery and induced NO release.

关 键 词：三甲氧基 苯甲酸酯 一氧化氮 内皮细胞 

分 类 号：R972[医药卫生—药品]