系统性红斑狼疮患者外周血调节性T细胞及TGF-β的检测  

Detection of Peripheral Blood T Cell and TGF-β in SLE

作　　者：董娟[1] 刘秀梅[1] 

机构地区：[1]山西医科大学附属第一医院,山西太原030001

出　　处：《中国现代医生》2010年第2期32-33,共2页China Modern Doctor

摘　　要：目的研究系统性红斑狼疮(SLE)患者外周血CD4+CD25+FOXP3+T细胞及细胞因子TGF-β的变化,分析其在SLE发病机制中的作用。方法收集SLE患者及健康对照组外周抗凝静脉血,流式细胞仪检测CD4+CD25+FOXP3+T细胞的表达。ELISA法检测研究对象血清中TGF-β的水平。结果SLE患者的CD4+CD25+FOXP3+T细胞百分率低于健康对照组,患者血清中TGF-β水平明显低于健康对照组(P<0.05)。结论CD4+CD25+FOXP3+T细胞数量减少和TGF-β含量降低可能与SLE发生有关。Objective To investigate the expression of peripheral blood CD4＋CD25＋ FOXP3＋T cells and the TGF-β level in SLE and analyze their role in the pathogenesis of SLE. Methods Peripheral anticoagulant venous blood was collected from SLE patients and healthy controls and the peripheral blood CD4^＋CD25^＋FOXP3^＋T cells were detected by flow cytometry and the peripheral blood TGF-β level was assayed by ELISA. Results The percentage of the CD4^＋CD25^＋FOXP3^＋T T cell was lower in the SLE patients than that in the healthy control group, and the TGF-β level in SLE was significantly higher than that in the healthy control group（P〈0.05）. Conclusion The reduction in the CD4^＋CD25^＋FOXP3^＋T cells and the lowered TGF- β may play an important role in the patbogenesis of SLE.

关 键 词：系统性红斑狼疮 调节性T细胞 TGF—β 

分 类 号：R714[医药卫生—妇产科学]