高速逆流色谱法对补骨脂中补骨脂素和异补骨脂素的分离纯化研究  

Preparative Iisolation and Purification of Psoralen and Isopsoralen from Psoralea Corylifolia by High-Speed Counter-Current Chromatography

作　　者：白鸽[1,2] 曹学丽[1,2] 谭莉[3] 唐亚林[3] 

机构地区：[1]北京工商大学化学与环境工程学院,北京100048 [2]北京市植物资源研究开发重点实验室,北京100048 [3]中国科学院化学研究所,北京100190

出　　处：《世界科学技术-中医药现代化》2009年第6期864-867,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金面上项目(B030107):以转铁蛋白受体介导的天然抗肿瘤化合物靶白给药物系相互作用的物理化学性质研究;负责人:唐亚林

摘　　要：补骨脂素和异补骨脂素是中药补骨脂的特征成分,含量较高且具有多种生物活性,目前已成为补骨脂及其产品的质量控制成分。本文以补骨脂干燥成熟果实为原料,经乙醇回流制备补骨脂粗提物,率先采用高速逆流色谱技术进行分离,所得样品由高效液相色谱检测纯度,并由对照品确定成分。实验结果表明:采用正己烷-乙酸乙酯-甲醇-水(7:3:5:5)作为两相溶剂体系,经一步逆流分离可以从补骨脂粗提物中分离得到补骨脂素和异补骨脂素,二者纯度分别达到99.4%、99.1%。该法具有制备量大、分离效率高等优点。High-speed counter-current chromatography （HSCCC） was employed for the separation of psoralen and isopsoralen from a crude extract of Psoralea corylifolia. The crude extract was obtained by extracting with ethanol from the dried seeds ofPsoralea corylifolia, n-Hexane-ethyl acetate-methanol-water （7 ： 3 ： 5 ： 5）was used as the HSCCC two-phase solvent system for the separation. The isolated high-pure components were analyzed by high performance liquid chro- matography （HPLC） and identified by comparing of the retention time with those of the standards. The results indicated that psoralen and isopsoralen were separated from other elements at 99.4% and 99.1% purity. High-speed counter-current chromatography could separate and prepare these two components successfully from Psoralea corylifolia seeds.

关 键 词：高速逆流色谱 补骨脂 补骨脂素 异补骨脂素 

分 类 号：R284.2[医药卫生—中药学] R286.0[医药卫生—中医学]