草莓组织培养与快繁技术研究  

Study on Strawberry's Tissue Culture and Rapid Propagation Technology

作　　者：孙敏杰 张艳秋 张晓燕 张天静 温健 

机构地区：[1]辽宁省经济作物科学研究所,辽宁辽阳111000

出　　处：《科技情报开发与经济》2009年第36期141-143,共3页Sci-Tech Information Development & Economy

摘　　要：以草莓品种"章姬"匍匐茎尖为外植体,采用添加不同生长素的MS培养基,对其进行组织培养研究。结果表明:草莓茎尖在MS+BA0.5mg/L+GA0.2mg/L培养基上能很好地诱导不定芽形成,诱导率95%;在继代增殖培养基MS+BA2mg/L+NAA0.1mg/L中增殖系数较高,为3.5;生根培养基为1/2MS+IBA0.5mg/L+活性炭3g/L生根率可达90%;将生根瓶苗炼苗一周,移植至草炭土和蛭石(2∶1)的穴盘内,移栽试管苗的成活率达90%以上,并且生长健壮。This paper takes the shoot tip of strawberry cultivar ZhangJi as an explants,and by using MS culture medium with different growth hormones,researches the tissue culture of strawberry.The results show that：MS＋BA0.5 mg/L＋GA0.2 mg/ L can induce adventitious buds well,and the rate of inducement is 95%;on subculture media MS＋BA2 mg/L＋NAA0.1 mg/L,the proliferation number is higher（3.5）;on root induction media 1/2MS＋IBA0.5 mg/L＋AC3 g/L,the root induction rate is 90%.Vitro plantlets are hardened off for 1 week,then are transplanted into the medium which is consisted of turfy soil and vermiculite（the ratio of turfy and vermiculite is 2∶1）;the survival rate is above 90%,and the plantlets grow well and vigorous.

关 键 词：草莓 匍匐茎尖 组织培养 快繁技术 

分 类 号：S668.4[农业科学—果树学] Q943.1[农业科学—园艺学]