系统性硬化症165例自身抗体及免疫球蛋白 补体检测分析  

Analysis of autoantibody,immunoglobulin and complement in serum from 165 cases of systemic sclerosis

作　　者：韦三华[1] 张静[1] 刘昕阳[1] 张婧[1] 黄晶[1] 张惠中[1] 

机构地区：[1]第四军医大学唐都医院临床实验与检验,输血科,西安710038

出　　处：《检验医学与临床》2010年第2期108-110,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨系统性硬化症患者血清自身抗体及免疫球蛋白、补体检测的临床意义。方法用免疫荧光法和免疫印迹法测定165例系统性硬化症患者血清自身抗体,散射免疫比浊法测定免疫球蛋白及补体,并对相关检查结果进行分析。结果165例系统性硬化症患者血清自身抗体测定中抗核抗体(ANA)阳性率为93.33%;ANA谱测定、抗硬皮病抗体70(抗-SCL-70)和抗着丝点抗体(抗-CENP-B)在弥散型和局限型硬化症患者中阳性率分别为65.17%和75.00%;血清免疫球蛋白测定以IgG升高为主,占69.70%,同时IgA和IgM也有不同程度升高,轻链λ和κ显著升高,分别占67.88%和64.24%;补体测定C3、C4有不同程度降低;其他检查可见单核细胞和球蛋白升高明显。结论系统性硬化症患者体内存在多种自身抗体,抗-SCL-70和抗-CENP-B是诊断系统性硬化症的标志抗体,患者体内出现明显体液免疫功能的紊乱,对诊断具有参考意义。Objective To investigate the clinical significance of autoantibody,immunoglobulin and complement detection in systemic sclerosis（SSc）. Methods To perform the detection of autoantibody with immunofluorescence and immunoblotting,and the determination of immunoglobulin and complement in serum from 165 cases of systemic sclerosis,and to conduct the statistical analysis of the results. Results For the detection of autoantibody,the positive rate of ANA was 93.33%. For the analysis of target antlgen,ttle positive rate of SCL 70 and CENP B was 65. 17% and 75.00% in dispersed sclerosis and circumscribed sclerosis respectively. The results of immunoglobulin and complement determination indicated that the level of immunoglobulin in SSc was higher than that of heathy blood donor, especially for IgG 69.70% ,but not for complement,which was lower than that of control. The levels of IgA and lgM increased with different extent. The levels of λ chain and κ chain were much higher than those of the control, which was 67.88% and 64.24% respectively. Levels of monocyte and globulin were obviously increased in the partial pa tients. Conclusion It is important for diagnosis of SSc to analyse autoantibodics,SCL-70 and CENP-B are marked autoantibodies. The hyperglobulinemia indicates the disorder of humoral immune function in SSc patients,it is helpful for diagnosis of SSc.

关 键 词：系统性硬化症 自身抗体 抗体.抗核 免疫球蛋白类 补体 

分 类 号：R392.11[医药卫生—免疫学] R593.25[医药卫生—基础医学]