染料木黄酮诱导人结肠癌SW480细胞凋亡及机制  

Apoptosis of Human Colorectal Cancer Cell SW480 Induced by Genistein and Its Molecular Mechanism

作　　者：蒋崇辉 童玲玲[2] 李纯颖[2] 李东阳[2] 

机构地区：[1]衡阳市中心医院药剂科,湖南衡阳421001 [2]南华大学公共卫生学院,湖南衡阳421001

出　　处：《湖南环境生物职业技术学院学报》2010年第1期19-22,共4页JOurnal of Hunan Environment Biological Polytechnic

摘　　要：目的探讨染料木黄酮(GEN)诱导人结肠癌SW480细胞凋亡的生物学效应及抗肿瘤机制.方法通过MTT法检测GEN对该细胞系生长的影响;应用PI染色流式细胞术(FCM)分析GEN处理前后的细胞周期分布的变化;SP免疫组化法检测细胞内bcl-2、Bax蛋白的表达.结果GEN能抑制SW480细胞增殖,呈浓度依赖性.流式细胞仪示不同浓度GEN作用于SW480细胞,出现G2/M阻滞;SP免疫组化结果表明GEN处理细胞72 h后,Bax蛋白表达升高,bcl-2蛋白表达下降.结论GEN有诱导人结肠癌SW480细胞凋亡的作用,其机制与bcl-2/Bax比值下降有关.图2,参12.Objective To study the effect of genistein （GEN） on human colorectal cancer cell line SW480, and to investigate the mechanisms of its antitumor effect. Methods SW480 ceils growth inhibition was measured by MTT assay. Cell cycle of SW480 cell treated with GEN in vitro was analyzed by flow cytometry. The protein levels of bcl- 2, Bax were determined by using immunohistoehemical technique. Results MTT assay showed that GEN might significantly inhibit proliferation of SW480 cell, in concentration - dependent manner. Flow cytometry analysis revealed that genistein arrested cell cycle in G2/M phase. SP immunohistochemistry revealed that the Bax expression was increased while bcl -2 expressed was decreased. Conclusion Genistein can significantly induce apoptosis of human colorectal cancer cell SW480, and the apoptosis of tumor cells is closely associated with down regulation of the ratio of bcl - 2/Bax. 2figs. , 12refs.

关 键 词：结肠肿瘤 染料木黄酮 细胞周期 凋亡 

分 类 号：R971.1[医药卫生—药品]