甘附冲剂抗大鼠心动过缓及其作用机制的研究  

The Study of Anti-bradycardic Effect and Mechanism of Ganfu Granule in Rats

作　　者：李东[1] 陈志武[2] 岑德意[2] 王迪生[3] 

机构地区：[1]徐州生物工程高等职业学校,江苏徐州221006 [2]安徽医科大学,安徽合肥230032 [3]蚌埠医学院第二附属医院,安徽蚌埠233040

出　　处：《中国实验方剂学杂志》2010年第1期68-71,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:探讨甘附冲剂抗大鼠心动过缓的可能作用机制。方法:用普萘洛尔(Pro)制备大鼠心率减慢模型;测定大鼠心肌组织中谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)的活性及丙二醛(MDA)的含量。放射免疫法测定心肌组织中环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)及血浆中降钙素基因相关肽(CGRP)、内皮素(ET)的含量。结果:30,90,270mg.kg-1甘附冲剂可显著抑制Pro引起的大鼠心率减慢(P<0.05或P<0.01),和心肌组织中MDA含量的升高,提高大鼠血浆CGRP的含量,并可显著降低心肌组织中cGMP的含量,提高cAMP/cGMP比值(P<0.05或P<0.01);90,270 mg.kg-1甘附冲剂可增强大鼠心肌组织中SOD和GSH-PX的活性(P<0.05);甘附冲剂各剂量对大鼠心肌组织中cAMP含量及大鼠血浆ET含量并没有明显影响。结论:甘附冲剂抗大鼠心动过缓的作用可能与抗自由基、升高CGRP及调节cAMP和cGMP的平衡有关。Objective： To study the anti-bradycardic mechanism of Ganfu granule in rats. Methods： The bradycardic model in rats was induced by propranolol （Pro）. the activity of glutathione peroxidase （GSH-PX） ,superoxide dismutase （SOD） and the content of Malonydialdehyde （MDA） in heart tissue were detected; the content of cyclic adenosine monophosphate （cAMP）, cyclic guanosine monophosphate （cGMP） in heart tissue as well as the content of calcitonin gene-related peptide （CGRP）,endothelin （ET） in plasma were detected by radioimmunoassay. Results.. Ganfu granule （30,90,270 mg·kg^-1） significantly inhibited the Pro-induced decrease of heart rate in rats, reduced the content of MDA,cGMP in heart tissue, markedly increased the content of CGRP in plasma and the ratio of cAMP/cGMP in heart tissue. Gan granule（90, 270 mg·kg^-1） markedly increased the activity of SOD and GSH-PX in heart tissue; Compare with model group, the changes of the content of ET in plasma and cAMP in heart tissue were not obviouse. Conclusion. The anti-bradycardic mechanism of Ganfu granule may be related to anti-flee radicals, increasing CGRP, regulating the balance between cAMP and cGMP.

关 键 词：心动过缓 自由基 降钙素基因相关肽 环磷酸腺苷 环磷酸鸟苷 

分 类 号：R285.5[医药卫生—中药学]