肿节风总黄酮对巨核系细胞体外扩增的作用研究  

Effects of Flavonoids of Sarcand Glabrain on the Expansion of Mature Megakaryocytes and Colony Forming Unit-megakaryocyte in vitro

作　　者：汤喜兰[1] 黄立新[2] 曾治君[1] 张启云[1] 单义民[1] 徐国良[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004 [2]江西艺术职业学院基础部,江西南昌330013

出　　处：《中国实验方剂学杂志》2010年第1期79-82,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江西省自然科学基金资助项目(0640161)

摘　　要：目的：探讨肿节风总黄酮（ZJF-HT）对小鼠骨髓巨核系细胞扩增的影响。方法：采用液体培养和半固体集落培养,观察ZJF-HT及其含药血清对成熟巨核细胞及巨核系祖细胞集落扩增的作用。结果：与模型组相比,ZJF-HT≥125μg.mL-1能使成熟巨核细胞数和巨核系祖细胞集落数增多（P〈0.05）;ZJF-HT含药血清在0.39%-6.25%体积浓度范围能增加成熟巨核细胞数,在0.20%-6.25%范围内能显著增加巨核系祖细胞集落数（P〈0.01）。结论：ZJF-HT及其含药血清均能促进巨核系细胞的体外扩增。Objective：To investigate the effects of the flavonoids of Sarcand glabrain（ZJF-HT） on the expansion of mature megakaryocytes and CFU-Meg in vitro. Methods. Megakaryocytes and megakaryocyte progenitor cells were cultured in liquid and semi-solid colony culture systems respectively. The effects of ZJF-HT and ZJF-HT serum on the expansion of mature megakaryocytes and CFU-Meg were observed. Results. ZJF-HT,up to 125 /2g·mL^-1 ,could increase the number of mature megakaryocytes and CFU-Meg compared with model group （P〈0.05）. ZJF-HT serum could significantly increase the number of mature megakaryocytes over the range of 0.39^- 6.25% and also significantly promote the formation of CFU-Meg over the range of 0.20%-6.25%（compared with model group, P〈0.01）. Conclusions. Both ZJF-HT and ZJF-HT serum can promote the expansion of mature megakaryocytes and CFU-Meg.

关 键 词：肿节风总黄酮 巨核细胞 扩增 

分 类 号：R285.5[医药卫生—中药学]